info:eu-repo/semantics/article
Purification and characterization of a GH43 β-xylosidase from Enterobacter sp. identified and cloned from forest soil bacteria
Fecha
2013-05Registro en:
Campos, Eleonora; Negro Alvarez, María José; Sabarís Di Lorenzo, Gonzalo Julián; González, Sergio Alberto; Rorig, Marcela; et al.; Purification and characterization of a GH43 β-xylosidase from Enterobacter sp. identified and cloned from forest soil bacteria; Elsevier Gmbh; Microbiological Research; 169; 2-3; 5-2013; 213-220
0944-5013
CONICET Digital
CONICET
Autor
Campos, Eleonora
Negro Alvarez, María José
Sabarís Di Lorenzo, Gonzalo Julián
González, Sergio Alberto
Rorig, Marcela
Talia, Paola
Grasso, Daniel Hector
Sáez, Felicia
Manzanares Secades, Paloma
Ballesteros Perdices, Mercedes
Cataldi, Ángel Adrián
Resumen
The use of lignocellulosic biomass for second generation biofuels requires optimization of enzymatic breakdown of plant cell walls. In this work, cellulolytic bacteria were isolated from a native and two cultivated forest soil samples. Amplification of glycosyl hydrolases was attempted by using a low stringency-degenerate primer PCR strategy, using total soil DNA and bulk DNA pooled from positive colonies as template. A set of primers was designed based on Acidothermus cellulolyticus genome, by search of conserved domains of glycosyl hydrolases (GH) families of interest. Using this approach, a fragment containing an open reading frame (ORF) with 98% identity to a putative GH43 beta-xylosidase coding gene from Enterobacter cloacae was amplified and cloned. The full protein was expressed in Escherichia coli as N-terminal or C-terminal His-tagged fusions and purified under native conditions. Only N-terminal fusion protein, His-Xyl43, presented beta-xylosidase activity. On pNPX, optimal activity was achieved at pH 6 and 40°C and Km and Kcat values were 2.92mM and 1.32seg-1, respectively. Activity was also demonstrated on xylobiose (X2), with Km 17.8mM and Kcat 380s-1. These results demonstrated that Xyl43 is a functional beta-xylosidase and it is the first evidence of this activity for Enterobacter sp.