info:eu-repo/semantics/article
Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
Fecha
2021-11-12Registro en:
Avila, Héctor Gabriel; Risso, Marikena Guadalupe; Cabrera, Marta; Ruybal, Paula; Repetto, Silvia Analía; et al.; Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples; Frontiers Media; Frontiers in Veterinary Science; 8; 12-11-2021; 1-8
2297-1769
2297-1769
CONICET Digital
CONICET
Autor
Avila, Héctor Gabriel
Risso, Marikena Guadalupe
Cabrera, Marta
Ruybal, Paula
Repetto, Silvia Analía
Butti, Marcos Javier
Trangoni, Marcos David
Santillan, Graciela Ines
Perez, Veronica Mirtha
Periago, Maria Victoria
Resumen
Ancylostoma caninum is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the cox-1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from A. caninum adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of A. caninum for epidemiological studies in endemic areas.