Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples

dc.creatorAvila, Héctor Gabriel
dc.creatorRisso, Marikena Guadalupe
dc.creatorCabrera, Marta
dc.creatorRuybal, Paula
dc.creatorRepetto, Silvia Analía
dc.creatorButti, Marcos Javier
dc.creatorTrangoni, Marcos David
dc.creatorSantillan, Graciela Ines
dc.creatorPerez, Veronica Mirtha
dc.creatorPeriago, Maria Victoria
dc.date.accessioned2022-08-10T03:01:40Z
dc.date.accessioned2022-10-15T06:23:07Z
dc.date.available2022-08-10T03:01:40Z
dc.date.available2022-10-15T06:23:07Z
dc.date.created2022-08-10T03:01:40Z
dc.date.issued2021-11-12
dc.identifierAvila, Héctor Gabriel; Risso, Marikena Guadalupe; Cabrera, Marta; Ruybal, Paula; Repetto, Silvia Analía; et al.; Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples; Frontiers Media; Frontiers in Veterinary Science; 8; 12-11-2021; 1-8
dc.identifier2297-1769
dc.identifierhttp://hdl.handle.net/11336/164832
dc.identifier2297-1769
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4354819
dc.description.abstractAncylostoma caninum is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the cox-1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from A. caninum adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of A. caninum for epidemiological studies in endemic areas.
dc.languageeng
dc.publisherFrontiers Media
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2021.770508/full
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fvets.2021.770508
dc.rightshttps://creativecommons.org/licenses/by/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectANCYLOSTOMA CANINUM
dc.subjectANCYLOSTOMIASIS
dc.subjectCOPRO-DIAGNOSIS
dc.subjectLOOP MEDIATED ISOTHERMAL AMPLIFICATION
dc.subjectMOLECULAR DIAGNOSIS
dc.titleDevelopment of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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