info:eu-repo/semantics/article
Multivariate analysis of organic acids in fermented food from reversed-phase high-performance liquid chromatography data
Fecha
2018-02Registro en:
Mortera, Pablo; Zuljan, Federico Alberto; Magni, Christian; Bortolato, Santiago Andres; Alarcon, Sergio Hugo; Multivariate analysis of organic acids in fermented food from reversed-phase high-performance liquid chromatography data; Elsevier Science; Talanta; 178; 2-2018; 15-23
0039-9140
CONICET Digital
CONICET
Autor
Mortera, Pablo
Zuljan, Federico Alberto
Magni, Christian
Bortolato, Santiago Andres
Alarcon, Sergio Hugo
Resumen
Multivariate calibration coupled to RP-HPLC with diode array detection (HPLC-DAD) was applied to the identification and the quantitative evaluation of the short chain organic acids (malic, oxalic, formic, lactic, acetic, citric, pyruvic, succinic, tartaric, propionic and α-cetoglutaric) in fermented food. The goal of the present study was to get the successful resolution of a system in the combined occurrence of strongly coeluting peaks, of distortions in the time sensors among chromatograms, and of the presence of unexpected compounds not included in the calibration step. Second-order HPLC-DAD data matrices were obtained in a short time (10 min) on a C18 column with a chromatographic system operating in isocratic mode (mobile phase was 20 mmol L−1 phosphate buffer at pH 2.20) and a flow-rate of 1.0 mL min−1 at room temperature. Parallel factor analysis (PARAFAC) and unfolded partial least-squares combined with residual bilinearization (U-PLS/RBL) were the second-order calibration algorithms select for data processing. The performance of the analytical parameters was good with an outstanding limit of detection (LODs) for acids ranging from 0.15 to 10.0 mmol L−1 in the validation samples. The improved method was applied to the analysis of many dairy products (yoghurt, cultured milk and cheese) and wine. The method was shown as an effective means for determining and following acid contents in fermented food and was characterized by reducibility with simple, high resolution and rapid procedure without derivatization of analytes.