info:eu-repo/semantics/article
Circadian structural plasticity drives remodeling of E Cell Output
Fecha
2020-12-21Registro en:
Duhart, José Manuel; Herrero, Anastasia; de la Cruz, Gabriel; Ispizua, Juan Ignacio; Pírez, Nicolas; et al.; Circadian structural plasticity drives remodeling of E Cell Output; Cell Press; Current Biology; 30; 24; 21-12-2020; 5040-5048
0960-9822
CONICET Digital
CONICET
Autor
Duhart, José Manuel
Herrero, Anastasia
de la Cruz, Gabriel
Ispizua, Juan Ignacio
Pírez, Nicolas
Ceriani, Maria Fernanda
Resumen
Behavioral outputs arise as a result of highly regulated yet flexible communication among neurons. The Drosophila circadian network includes 150 neurons that dictate the temporal organization of locomotor activity; under light-dark (LD) conditions, flies display a robust bimodal pattern. The pigment-dispersing factor (PDF)-positive small ventral lateral neurons (sLNv) have been linked to the generation of the morning activity peak (the “M cells”), whereas the Cryptochrome (CRY)-positive dorsal lateral neurons (LNds) and the PDF-negative sLNv are necessary for the evening activity peak (the “E cells”) [1, 2]. While each group directly controls locomotor output pathways [3], an interplay between them along with a third dorsal cluster (the DN1ps) is necessary for the correct timing of each peak and for adjusting behavior to changes in the environment [4–7]. M cells set the phase of roughly half of the circadian neurons (including the E cells) through PDF [5, 8–10]. Here, we show the existence of synaptic input provided by the evening oscillator onto the M cells. Both structural and functional approaches revealed that E-to-M cell connectivity changes across the day, with higher excitatory input taking place before the day-to-night transition. We identified two different neurotransmitters, acetylcholine and glutamate, released by E cells that are relevant for robust circadian output. Indeed, we show that acetylcholine is responsible for the excitatory input from E cells to M cells, which show preferential responsiveness to acetylcholine during the evening. Our findings provide evidence of an excitatory feedback between circadian clusters and unveil an important plastic remodeling of the E cells’ synaptic connections.