dc.creator | Duhart, José Manuel | |
dc.creator | Herrero, Anastasia | |
dc.creator | de la Cruz, Gabriel | |
dc.creator | Ispizua, Juan Ignacio | |
dc.creator | Pírez, Nicolas | |
dc.creator | Ceriani, Maria Fernanda | |
dc.date.accessioned | 2021-10-02T01:42:35Z | |
dc.date.accessioned | 2022-10-15T02:18:29Z | |
dc.date.available | 2021-10-02T01:42:35Z | |
dc.date.available | 2022-10-15T02:18:29Z | |
dc.date.created | 2021-10-02T01:42:35Z | |
dc.date.issued | 2020-12-21 | |
dc.identifier | Duhart, José Manuel; Herrero, Anastasia; de la Cruz, Gabriel; Ispizua, Juan Ignacio; Pírez, Nicolas; et al.; Circadian structural plasticity drives remodeling of E Cell Output; Cell Press; Current Biology; 30; 24; 21-12-2020; 5040-5048 | |
dc.identifier | 0960-9822 | |
dc.identifier | http://hdl.handle.net/11336/142342 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4334332 | |
dc.description.abstract | Behavioral outputs arise as a result of highly regulated yet flexible communication among neurons. The Drosophila circadian network includes 150 neurons that dictate the temporal organization of locomotor activity; under light-dark (LD) conditions, flies display a robust bimodal pattern. The pigment-dispersing factor (PDF)-positive small ventral lateral neurons (sLNv) have been linked to the generation of the morning activity peak (the “M cells”), whereas the Cryptochrome (CRY)-positive dorsal lateral neurons (LNds) and the PDF-negative sLNv are necessary for the evening activity peak (the “E cells”) [1, 2]. While each group directly controls locomotor output pathways [3], an interplay between them along with a third dorsal cluster (the DN1ps) is necessary for the correct timing of each peak and for adjusting behavior to changes in the environment [4–7]. M cells set the phase of roughly half of the circadian neurons (including the E cells) through PDF [5, 8–10]. Here, we show the existence of synaptic input provided by the evening oscillator onto the M cells. Both structural and functional approaches revealed that E-to-M cell connectivity changes across the day, with higher excitatory input taking place before the day-to-night transition. We identified two different neurotransmitters, acetylcholine and glutamate, released by E cells that are relevant for robust circadian output. Indeed, we show that acetylcholine is responsible for the excitatory input from E cells to M cells, which show preferential responsiveness to acetylcholine during the evening. Our findings provide evidence of an excitatory feedback between circadian clusters and unveil an important plastic remodeling of the E cells’ synaptic connections. | |
dc.language | eng | |
dc.publisher | Cell Press | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/current-biology/fulltext/S0960-9822(20)31427-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982220314275%3Fshowall%3Dtrue | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.cub.2020.09.057 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | circadian network | |
dc.subject | fast neurotransmission | |
dc.subject | M oscillator | |
dc.subject | E oscillator | |
dc.title | Circadian structural plasticity drives remodeling of E Cell Output | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |