Role of allopregnanolone on cerebellar granule cells neurogenesis

Keller, Elizabeth Ana; Zamparini, A.; Borodinsky, L.N.; Gravielle, Maria Clara; Fiszman, Monica Lidia

info:eu-repo/semantics/article

Fecha

2004-10

Registro en:

Keller, Elizabeth Ana; Zamparini, A.; Borodinsky, L.N.; Gravielle, Maria Clara; Fiszman, Monica Lidia; Role of allopregnanolone on cerebellar granule cells neurogenesis; Elsevier; Developmental Brain Research; 153; 1; 10-2004; 13-17

0165-3806

http://hdl.handle.net/11336/94394

CONICET Digital

CONICET

https://repositorioslatinoamericanos.uchile.cl/handle/2250/4329372

Autor

Keller, Elizabeth Ana

Zamparini, A.

Borodinsky, L.N.

Gravielle, Maria Clara

Fiszman, Monica Lidia

Institución

Consejo Nacional de Investigaciones Científicas y Tecnológicas (Argentina)

Resumen

The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1–1 μM) increased [3H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl2, 10 μM nifedipine, 10 μM picrotoxin or by 50 μM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABAA receptors activation.

Materias

CALCIUM

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