info:eu-repo/semantics/article
Contribution of neural crest and GLAST+ Wnt1+ bone marrow pericytes with liver fibrogenesis and/or regeneration
Fecha
2020-02Registro en:
Sierra, Romina; Gomez Bustillo, Sofia; Kameneva, Polina; Fiore, Esteban Juan; Mazzone, Graciela Luján; et al.; Contribution of neural crest and GLAST+ Wnt1+ bone marrow pericytes with liver fibrogenesis and/or regeneration; Wiley Blackwell Publishing, Inc; Liver International; 40; 4; 2-2020; 977-987
1478-3223
CONICET Digital
CONICET
Autor
Sierra, Romina
Gomez Bustillo, Sofia
Kameneva, Polina
Fiore, Esteban Juan
Mazzone, Graciela Luján
Borda Acevedo, Franco Maximiliano
Blanco, María V
Usuardi, Chiara
Furlan, Alessandro
Ernfors, Patrik
Alaniz, Laura Daniela
Montaner, Alejandro Daniel
Adameyko, Igor
Aquino, Jorge Benjamin
Resumen
Background and aims: Liver fibrosis results from cycles of liver damage and scar formation. We herein aimed at analysing neural crest cells and/or bone marrow stromal cells contribution to the liver. Methods: Two liver fibrosis and one hepatectomy model were applied on double-transgenic loxP-Cre mouse lines. Results: Increased numbers of glia with more complex processes were found in fibrotic livers. During embryonic development, only few cells were traced in the liver and bone marrow, in a minor fraction of mice of different neural crest reporter strains analysed: therefore, a neural crest origin of such cells is doubtful. In the fibrotic liver, a significantly higher incidence of endothelial cells and hepatocyte-like cells expressing the reporter gene Tomato were found in Wnt1-Cre-Tom and GLAST-CreERT2-Tom mice. Consistently, during early fibrogenesis stromal Wnt1-traced cells, with progenitor (CFU-F) properties, get likely mobilized to peripheral blood. Circulating adult Wnt1-traced cells are stromal cells and lack from the expression of other bone marrow and endothelial progenitor cells markers. Furthermore, in a 70% hepatectomy model GLAST+ Wnt1-traced pericytes were found to be mobilized from the bone marrow and the incidence of GLAST-traced hepatocyte-like cells was increased. Finally, GLAST-traced hepatocyte like-cells were found to maintain the expression of stromal markers. Conclusions: Our data suggest a gliosis process during liver fibrogenesis. While neural crest cells probably do not contribute with other liver cell types than glia, GLAST+ Wnt1-traced bone marrow pericytes are likely a source of endothelial and hepatocyte-like cells after liver injury and do not contribute to scarring.