dc.creator | Sierra, Romina | |
dc.creator | Gomez Bustillo, Sofia | |
dc.creator | Kameneva, Polina | |
dc.creator | Fiore, Esteban Juan | |
dc.creator | Mazzone, Graciela Luján | |
dc.creator | Borda Acevedo, Franco Maximiliano | |
dc.creator | Blanco, María V | |
dc.creator | Usuardi, Chiara | |
dc.creator | Furlan, Alessandro | |
dc.creator | Ernfors, Patrik | |
dc.creator | Alaniz, Laura Daniela | |
dc.creator | Montaner, Alejandro Daniel | |
dc.creator | Adameyko, Igor | |
dc.creator | Aquino, Jorge Benjamin | |
dc.date.accessioned | 2022-08-01T14:49:08Z | |
dc.date.accessioned | 2022-10-14T23:14:58Z | |
dc.date.available | 2022-08-01T14:49:08Z | |
dc.date.available | 2022-10-14T23:14:58Z | |
dc.date.created | 2022-08-01T14:49:08Z | |
dc.date.issued | 2020-02 | |
dc.identifier | Sierra, Romina; Gomez Bustillo, Sofia; Kameneva, Polina; Fiore, Esteban Juan; Mazzone, Graciela Luján; et al.; Contribution of neural crest and GLAST+ Wnt1+ bone marrow pericytes with liver fibrogenesis and/or regeneration; Wiley Blackwell Publishing, Inc; Liver International; 40; 4; 2-2020; 977-987 | |
dc.identifier | 1478-3223 | |
dc.identifier | http://hdl.handle.net/11336/163680 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4318250 | |
dc.description.abstract | Background and aims: Liver fibrosis results from cycles of liver damage and scar formation. We herein aimed at analysing neural crest cells and/or bone marrow stromal cells contribution to the liver. Methods: Two liver fibrosis and one hepatectomy model were applied on double-transgenic loxP-Cre mouse lines. Results: Increased numbers of glia with more complex processes were found in fibrotic livers. During embryonic development, only few cells were traced in the liver and bone marrow, in a minor fraction of mice of different neural crest reporter strains analysed: therefore, a neural crest origin of such cells is doubtful. In the fibrotic liver, a significantly higher incidence of endothelial cells and hepatocyte-like cells expressing the reporter gene Tomato were found in Wnt1-Cre-Tom and GLAST-CreERT2-Tom mice. Consistently, during early fibrogenesis stromal Wnt1-traced cells, with progenitor (CFU-F) properties, get likely mobilized to peripheral blood. Circulating adult Wnt1-traced cells are stromal cells and lack from the expression of other bone marrow and endothelial progenitor cells markers. Furthermore, in a 70% hepatectomy model GLAST+ Wnt1-traced pericytes were found to be mobilized from the bone marrow and the incidence of GLAST-traced hepatocyte-like cells was increased. Finally, GLAST-traced hepatocyte like-cells were found to maintain the expression of stromal markers. Conclusions: Our data suggest a gliosis process during liver fibrogenesis. While neural crest cells probably do not contribute with other liver cell types than glia, GLAST+ Wnt1-traced bone marrow pericytes are likely a source of endothelial and hepatocyte-like cells after liver injury and do not contribute to scarring. | |
dc.language | eng | |
dc.publisher | Wiley Blackwell Publishing, Inc | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/liv.14401 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1111/liv.14401 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | CIRRHOSIS | |
dc.subject | ENDOTHELIAL CELLS | |
dc.subject | HEPATECTOMY | |
dc.subject | HEPATOCYTE-LIKE CELLS | |
dc.subject | MESENCHYMAL STEM CELLS | |
dc.subject | PLASTICITY | |
dc.title | Contribution of neural crest and GLAST+ Wnt1+ bone marrow pericytes with liver fibrogenesis and/or regeneration | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |