info:eu-repo/semantics/article
Biotransformation of cladribine using a stabilized biocatalyst in calcium alginate beads
Fecha
2019-10Registro en:
Lapponi, María José; Britos, Claudia Noelia; Rivero, Cintia Wanda; Trelles, Jorge Abel; Biotransformation of cladribine using a stabilized biocatalyst in calcium alginate beads; American Institute of Chemical Engineers; Biotechnology Progress; 36; 2; 10-2019; 1-6
8756-7938
CONICET Digital
CONICET
Autor
Lapponi, María José
Britos, Claudia Noelia
Rivero, Cintia Wanda
Trelles, Jorge Abel
Resumen
Cladribine is a nucleoside analogue widely used in the pharmaceutical industry for the treatment of several neoplasms, including hairy-cell leukemia among others. This compound has also shown efficacy in the treatment of autoimmune diseases such as rheumatoid arthritis and multiple sclerosis. In this work, a green bioprocess for cladribine biosynthesis using immobilized Arthrobacter oxydans was developed. The microorganism was stabilized by entrapment immobilization in the natural matrix alginate. Different reaction parameters were optimized obtaining a biocatalyst able to achieve cladribine bioconversion values close to 85% after 1 hr, the shortest reaction times reported so far. The developed bioprocess was successfully scaled-up reaching a productivity of 138 mg L−1 hr−1. Also, the biocatalyst was stable for 5 months in storage and in 96 hr at operational conditions.