dc.creator | Lapponi, María José | |
dc.creator | Britos, Claudia Noelia | |
dc.creator | Rivero, Cintia Wanda | |
dc.creator | Trelles, Jorge Abel | |
dc.date.accessioned | 2022-07-28T13:08:45Z | |
dc.date.accessioned | 2022-10-14T21:50:32Z | |
dc.date.available | 2022-07-28T13:08:45Z | |
dc.date.available | 2022-10-14T21:50:32Z | |
dc.date.created | 2022-07-28T13:08:45Z | |
dc.date.issued | 2019-10 | |
dc.identifier | Lapponi, María José; Britos, Claudia Noelia; Rivero, Cintia Wanda; Trelles, Jorge Abel; Biotransformation of cladribine using a stabilized biocatalyst in calcium alginate beads; American Institute of Chemical Engineers; Biotechnology Progress; 36; 2; 10-2019; 1-6 | |
dc.identifier | 8756-7938 | |
dc.identifier | http://hdl.handle.net/11336/163379 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4310588 | |
dc.description.abstract | Cladribine is a nucleoside analogue widely used in the pharmaceutical industry for the treatment of several neoplasms, including hairy-cell leukemia among others. This compound has also shown efficacy in the treatment of autoimmune diseases such as rheumatoid arthritis and multiple sclerosis. In this work, a green bioprocess for cladribine biosynthesis using immobilized Arthrobacter oxydans was developed. The microorganism was stabilized by entrapment immobilization in the natural matrix alginate. Different reaction parameters were optimized obtaining a biocatalyst able to achieve cladribine bioconversion values close to 85% after 1 hr, the shortest reaction times reported so far. The developed bioprocess was successfully scaled-up reaching a productivity of 138 mg L−1 hr−1. Also, the biocatalyst was stable for 5 months in storage and in 96 hr at operational conditions. | |
dc.language | eng | |
dc.publisher | American Institute of Chemical Engineers | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2927 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/btpr.2927 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | 2-CHLORODEOXYADENOSINE | |
dc.subject | ANTITUMOR AGENT | |
dc.subject | BIOTRANSFORMATIONS | |
dc.subject | GREEN CHEMISTRY | |
dc.subject | WHOLE-CELL IMMOBILIZATION | |
dc.title | Biotransformation of cladribine using a stabilized biocatalyst in calcium alginate beads | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |