This paper presents the validation of the Entrepreneurial Intention Questionnaire (EIQ) in Colombia. This questionnaire, developed and validated in Spain (Rueda, Moriano, & Liñán, 2015), draws on the Theory of Planned Behaviour (TPB, Ajzen, 1991). The TPB is the most widely used model for predicting entrepreneurial intention nowadays. With a sample of 316 Colombian university students, the results of the structural equation modeling confirm that the EIQ has a high reliability and predictive validity of entrepreneurial intentions. The availability of reliable measurement scales validated in different cultural contexts allows comparisons among institutions and/or within an institution along the time (e.g. before and after a course on entrepreneurship). A deep understanding of the psychosocial antecedents (i.e. attitudes toward entrepreneurship, subjective norms, and entrepreneurial self-efficacy) that lead young university students to become entrepreneurs might contribute to a better design of the entrepreneurship education programmes that are being developed from universities and other institutions.