Artículo de revista
EPA/DHA concentrate by urea complexation decreases hyperinsulinemia and increases Plin5 in the liver of mice fed a high-fat diet
Fecha
2020Registro en:
Molecules 2020, 25, 3289
10.3390/molecules25143289
Autor
Espinosa Escalona, Berta
Ross, Andrés
Dovale Rosabal, Gretel
Pino de la Fuente, Francisco
Uribe Oporto, Ernesto
Sacristán Inostroza, Camila
Ruiz Rojas, Paulina
Valenzuela Báez, Rodrigo
Romero Palacios, Nalda
Aubourg, Santiago P.
Rodríguez Melis, Alicia
Institución
Resumen
Dietary intake of eicosapentaenoic/docosahexaenoic acid (EPA/DHA) reduces insulin
resistance and hepatic manifestations through the regulation of metabolism in the liver. Obese mice
present insulin resistance and lipid accumulation in intracellular lipid droplets (LDs). LD-associated
proteins perilipin (Plin) have an essential role in both adipogenesis and lipolysis; Plin5 regulates
lipolysis and thus contributes to fat oxidation. The purpose of this study was to compare the effects
of deodorized refined salmon oil (DSO) and its polyunsaturated fatty acids concentrate (CPUFA)
containing EPA and DHA, obtained by complexing with urea, on obesity-induced metabolic alteration.
CPUFA maximum content was determined using the Box–Behnken experimental design based on
Surface Response Methodology. The optimized CPUFA was administered to high-fat diet (HFD)-fed
mice (200 mg/kg/day of EPA + DHA) for 8 weeks. No significant differences (p > 0.05) in cholesterol,
glycemia, LDs or transaminase content were found. Fasting insulin and hepatic Plin5 protein level
increased in the group supplemented with the EPA + DHA optimized product (38.35 g/100 g total fatty
acids) compared to obese mice without fish oil supplementation. The results suggest that processing
salmon oil by urea concentration can generate an EPA+DHA dose useful to prevent the increase of
fasting insulin and the decrease of Plin5 in the liver of insulin-resistant mice.