Artículo de revista
Overexpression of MMPs, cytokines, and RANKL/OPG in temporomandibular joint osteoarthritis and their association with joint pain, mouth opening, and bone degeneration: A preliminary report
Fecha
2020Registro en:
Oral Diseases Sep 2020
10.1111/odi.13623
Autor
Cafferata Chea, Emilio
Monasterio Ocares, Gustavo
Castillo Franzoy, Francisca
Carvajal Pavez, Paola
Flores Sepúlveda, Guillermo
Díaz Guzmán, Walter
Fuentes del Campo, Aler
Vernal Astudillo, Rolando
Institución
Resumen
Objective This study aimed to determine the expression of distinct matrix metalloproteinases, cytokines, and bone resorptive factors in temporomandibular joint osteoarthritis (TMJ-OA) patients and their association with joint pain, mouth opening, and subchondral bone degeneration. Materials and methods Twelve patients affected with TMJ-OA (n = 5), disk displacement without reduction (DDWoR) (n = 3), or disk displacement with reduction (DDWR) (n = 4) were selected. Joint pain was quantified by using visual analog scale, mouth opening was quantified at the maximum pain-free aperture, and bone degeneration was quantified using joint imaging. Synovial fluid samples were collected and immediately processed for cell and synovial fluid recovering. From cells, the MMP-1, MMP-2, MMP-8, MMP-13, IL-6, IL-23, and TNF-alpha expression was quantified by qPCR. From synovial fluid, the RANKL and OPG levels were quantified by ELISA. Results Higher levels of MMP-1, MMP-8, MMP-13, IL-6, IL-23, TNF-alpha, and RANKL/OPG ratio were detected in TMJ-OA compared with DDWoR and DDWR patients (p < .05). Joint pain significantly correlated with TNF-alpha levels (r = .975,p = .029). Besides, imaging signs of bone degeneration significantly correlated with RANKL/OPG ratio (r = .949,p = .042). Conversely, mouth opening did not correlate with any of the analyzed mediators. Conclusion During TMJ-OA, a pathological response characterized by the overexpression of TNF-alpha and RANKL/OPG could be involved in joint pain and subchondral bone degeneration.