Artículo de revista
Poly-epsilon-caprolactone Nanoparticles Loaded with 4-Nerolidylcatechol (4-NC) for Growth Inhibition of Microsporum canis
Fecha
2020Registro en:
Antibiotics 2020, 9, 894
10.3390/antibiotics9120894
Autor
Greatti, Vanessa Raquel
Oda, Fernando
Sorrechia, Rodrigo
Kapp, Bárbara Regina
Seraphim, Carolina Manzato
Villas Boas Weckwerth, Ana Carolina
Chorilli, Marlus
Da Silva, Patricia Bento
Eloy, Josimar O.
Kogan, Marcelo J.
Morales Montecinos, Javier
Linhari Rodrigues Pietro, Rosemeire Cristina
Institución
Resumen
Dermatophyte fungal infections are difficult to treat because they need long-term treatments. 4-Nerolidylcatechol (4-NC) is a compound found in Piper umbellatum that has been reported to demonstrate significant antifungal activity, but is easily oxidizable. Due to this characteristic, the incorporation in nanostructured systems represents a strategy to guarantee the compound's stability compared to the isolated form and the possibility of improving antifungal activity. The objective of this study was to incorporate 4-NC into polymeric nanoparticles to evaluate, in vitro and in vivo, the growth inhibition of Microsporum canis. 4-NC was isolated from fresh leaves of P. umbellatum, and polymer nanoparticles of polycaprolactone were developed by nanoprecipitation using a 1:5 weight ratio (drug:polymer). Nanoparticles exhibited excellent encapsulation efficiency, and the antifungal activity was observed in nanoparticles with 4-NC incorporated. Polymeric nanoparticles can be a strategy employed for decreased cytotoxicity, increasing the stability and solubility of substances, as well as improving the efficacy of 4-NC.