Dissertação
Caracterização físico-química do mel do bioma pampa brasileiro e estudo do seu potencial antioxidante in vitro e in vivo em modelo de Drosophila melanogaster
Fecha
2014-03-14Registro en:
CRUZ, Litiele Cezar da. PHYSICOCHEMICAL CHARACTERIZATION OF BRAZILIAN PAMPA BIOME
HONEY AND STUDY OF ITS ANTIOXIDANT POTENTIAL in vitro AND in vivo IN Drosophila melanogaster MODEL. 2014. 66 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.
Autor
Cruz, Litiele Cezar da
Institución
Resumen
Honey is a complex mixture produced by honey bees from the nectar, which is used by
its sweetening properties, as well as by its human health benefits. The aim of this study was to
characterize, for the first time in literature, the Brazilian Pampa Biome honey in terms of
quality parameters and determination of its antioxidant properties in vitro and in vivo. A total
of 10 honey samples were tested for physicalchemical parameters, as moisture, free acidity,
reducing sugars, hydroxymethylfurfural (HMF) levels, among others. The antioxidant activity
(in vitro), as evaluated by total phenolics, flavonoid content, FRAP and DPPH-ABTS
scavenging activity. Drosophila melanogaster (in vivo) were exposed to oxidative stress
induced by Iron (Fe) and Paraquat (PQ) in different treatment protocols, in the presence or
absence of honey. The survivorship and locomotor activity (negative geotaxis) were analyzed.
Possible glycemic alterations on honey diet were also evaluated. The results of
physicochemical analysis indicated that all honeys were in accordance with the standards
established by the Brazilian law, in which follows international standards. All honey samples
showed significant antioxidant activity in vitro. Flies treated with honey showed increased
lifespan and were protected against oxidative stress induced by Fe and PQ in the different
treatment protocols (48h and 7 days), in regards of mortality and locomotor deficits. Despite
the high sugar content of honey, glucose content were unchanged in honey fed flies, when
compared to flies fed on honey-equivalent amounts of sugars. This study demonstrates that
Brazilian Pampa Biome honey has a high quality level, a significant antioxidant activity in
vitro and a protective potential against oxidative stress (in vivo). It has been also demonstrated
that Drosophila melanogaster could be a valid model for studies with honey, as well as the
usage of this natural product as an alternative in the therapy of oxidative stress-associated
diseases.