Artículos de revistas
Deficient Vitamin E Uptake during Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI
Fecha
2017Registro en:
Scientific Reports, Volumen 7, Issue 1, 2018,
20452322
10.1038/s41598-017-05422-w
Autor
Santander, Nicolás
Lizama, Carlos
Parga, María José
Quiroz, Alonso
Pérez, Druso
Echeverría, Guadalupe
Ulloa, Lorena
Palma, Verónica
Rigotti, Attilio
Busso, Dolores
Institución
Resumen
© 2017 The Author(s).SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and Vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI-/- embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic Vitamin E uptake during murine neural tube closure. Our results showed that SR-BI-/- embryos had a very low Vitamin E content in comparison to SR-BI+/+ embryos. Whereas SR-BI-/- embryos with closed neural tubes (nSR-BI-/-) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI+/+ and nSR-BI-/- embryos were detected in SR-BI-/- with NTD (NTD SR-BI-/-). Reduced expression of Pax3, Alx1 and Alx3 genes was found in N