Artículos de revistas
Interleukin-6 and neuregulin-1 as regulators of utrophin expression via the activation of NRG-1/ErbB signaling pathway in mdx cells
Fecha
2017Registro en:
Biochimica et Biophysica Acta - Molecular Basis of Disease, Volumen 1863, Issue 3, 2018, Pages 770-780
1879260X
09254439
10.1016/j.bbadis.2016.12.008
Autor
Juretić, Nevenka
Díaz, Josefina
Romero, Felipe
González, Gustavo
Jaimovich Pérez, Enrique
Riveros, Nora
Institución
Resumen
© 2016 Elsevier B.V. Duchenne muscular dystrophy (DMD) is a neuromuscular disease originated by mutations in the dystrophin gene. A promising therapeutic approach deals with functional substitution of dystrophin by utrophin, a structural homolog that might be able to compensate dystrophin absence in DMD muscle fibers. It has been described that both interleukin-6 (IL-6) and neuregulin-1 (NRG-1; Heregulin-HRG) induce utrophin expression in skeletal muscle. We investigated a possible functional link among IL-6, NRG-1 and utrophin, in normal (C57) and dystrophic (mdx) skeletal muscle cells. Western Blot analysis allowed us to demonstrate that IL-6 (100 ng/mL) induces NRG-1 receptor phosphorylation (ErbB2/ErbB3) in both cell types, in a process that depends on intracellular Ca2 + and metalloproteinase activity; it also induces a transient increase of ERK1 and GABPα phosphorylation only in dystrophic myotubes. Semiquantitative PCR showed that IL-6 treatment increases utrophin mRNA levels ju