Artículo de revista
High-frequency field stimulation of primary neurons enhances ryanodine receptor-mediated Ca2+ release and generates hydrogen peroxide, which jointly stimulate NF-κB activity
Fecha
2011Registro en:
Antioxidants and Redox Signaling, Volumen 14, Issue 7, 2018, Pages 1245-1259
15230864
10.1089/ars.2010.3238
Autor
Riquelme, Denise
Alvarez, Alvaro
Leal, Nancy
Adasme, Tatiana
Espinoza, Italo
Valdés, Juan Antonio
Troncoso, Natalia
Hartel, Steffen
Segura Hidalgo, Jorge Antonio
Hidalgo Tapia, María Cecilia
Carrasco, M. Angélica
Institución
Resumen
Neuronal electrical activity increases intracellular Ca2+ concentration and generates reactive oxygen species. Here, we show that high frequency field stimulation of primary hippocampal neurons generated Ca 2+ signals with an early and a late component, and promoted hydrogen peroxide generation via a neuronal NADPH oxidase. Hydrogen peroxide generation required both Ca2+ entry through N-methyl-D-aspartate receptors and Ca2+ release mediated by ryanodine receptors (RyR). Field stimulation also enhanced nuclear translocation of the NF-κB p65 protein and NF-κB -dependent transcription, and increased c-fos mRNA and type-2 RyR protein content. Preincubation with inhibitory ryanodine or with the antioxidant N-acetyl L-cysteine abolished the increase in hydrogen peroxide generation and the late Ca2+ signal component induced by electrical stimulation. Primary cortical cells behaved similarly as primary hippocampal cells. Exogenous hydrogen peroxide also activated NF-κB-dependent transcription