dc.creator | Riquelme, Denise | |
dc.creator | Alvarez, Alvaro | |
dc.creator | Leal, Nancy | |
dc.creator | Adasme, Tatiana | |
dc.creator | Espinoza, Italo | |
dc.creator | Valdés, Juan Antonio | |
dc.creator | Troncoso, Natalia | |
dc.creator | Hartel, Steffen | |
dc.creator | Segura Hidalgo, Jorge Antonio | |
dc.creator | Hidalgo Tapia, María Cecilia | |
dc.creator | Carrasco, M. Angélica | |
dc.date.accessioned | 2019-03-11T13:01:00Z | |
dc.date.available | 2019-03-11T13:01:00Z | |
dc.date.created | 2019-03-11T13:01:00Z | |
dc.date.issued | 2011 | |
dc.identifier | Antioxidants and Redox Signaling, Volumen 14, Issue 7, 2018, Pages 1245-1259 | |
dc.identifier | 15230864 | |
dc.identifier | 10.1089/ars.2010.3238 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/165200 | |
dc.description.abstract | Neuronal electrical activity increases intracellular Ca2+ concentration and generates reactive oxygen species. Here, we show that high frequency field stimulation of primary hippocampal neurons generated Ca 2+ signals with an early and a late component, and promoted hydrogen peroxide generation via a neuronal NADPH oxidase. Hydrogen peroxide generation required both Ca2+ entry through N-methyl-D-aspartate receptors and Ca2+ release mediated by ryanodine receptors (RyR). Field stimulation also enhanced nuclear translocation of the NF-κB p65 protein and NF-κB -dependent transcription, and increased c-fos mRNA and type-2 RyR protein content. Preincubation with inhibitory ryanodine or with the antioxidant N-acetyl L-cysteine abolished the increase in hydrogen peroxide generation and the late Ca2+ signal component induced by electrical stimulation. Primary cortical cells behaved similarly as primary hippocampal cells. Exogenous hydrogen peroxide also activated NF-κB-dependent transcription | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Antioxidants and Redox Signaling | |
dc.subject | Biochemistry | |
dc.subject | Physiology | |
dc.subject | Molecular Biology | |
dc.subject | Clinical Biochemistry | |
dc.subject | Cell Biology | |
dc.title | High-frequency field stimulation of primary neurons enhances ryanodine receptor-mediated Ca2+ release and generates hydrogen peroxide, which jointly stimulate NF-κB activity | |
dc.type | Artículo de revista | |