Artículo de revista
Biosynthesis of monoterpene hydrocarbons from [1-3H]neryl pyrophosphate and [1-3H]geranyl pyrophosphate by soluble enzymes from Citrus limonum
Fecha
1977Registro en:
Archives of Biochemistry and Biophysics, Volumen 180, Issue 2, 2018, Pages 318-327
10960384
00039861
10.1016/0003-9861(77)90044-3
Autor
Chayet, Liliana
Rojas, Cecilia
Cardemil, Cecilia
Jabalquinto, Ana María
Vicuña, Ana María
Cori, Ana María
Institución
Resumen
A soluble enzyme preparation from the flavedo of Citrus limonum transforms [1-3H1]neryl pyrophosphate or [1-3H1]geranyl pyrophosphate into β-pinene, sabinene, α-pinene, and limonene. The enzyme has been partially purified and stabilized by precipitation with polyethyleneglycol. The enzymic cyclization requires the presence of Mn2+, which cannot be replaced with Mg2+. The addition of reagents containing sulfhydryl groups is essential for optimal activity. Allylic C10 monophosphates do not act as substrates, but they inhibit hydrocarbon formation. Inorganic pyrophosphate has a similar inhibitory effect. No interconversion of neryl and geranyl pyrophosphate has been observed. Possible pathways for the enzymic cyclization reactions are proposed. © 1977.