dc.creator | Chayet, Liliana | |
dc.creator | Rojas, Cecilia | |
dc.creator | Cardemil, Cecilia | |
dc.creator | Jabalquinto, Ana María | |
dc.creator | Vicuña, Ana María | |
dc.creator | Cori, Ana María | |
dc.date.accessioned | 2018-12-20T14:08:10Z | |
dc.date.available | 2018-12-20T14:08:10Z | |
dc.date.created | 2018-12-20T14:08:10Z | |
dc.date.issued | 1977 | |
dc.identifier | Archives of Biochemistry and Biophysics, Volumen 180, Issue 2, 2018, Pages 318-327 | |
dc.identifier | 10960384 | |
dc.identifier | 00039861 | |
dc.identifier | 10.1016/0003-9861(77)90044-3 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/154123 | |
dc.description.abstract | A soluble enzyme preparation from the flavedo of Citrus limonum transforms [1-3H1]neryl pyrophosphate or [1-3H1]geranyl pyrophosphate into β-pinene, sabinene, α-pinene, and limonene. The enzyme has been partially purified and stabilized by precipitation with polyethyleneglycol. The enzymic cyclization requires the presence of Mn2+, which cannot be replaced with Mg2+. The addition of reagents containing sulfhydryl groups is essential for optimal activity. Allylic C10 monophosphates do not act as substrates, but they inhibit hydrocarbon formation. Inorganic pyrophosphate has a similar inhibitory effect. No interconversion of neryl and geranyl pyrophosphate has been observed. Possible pathways for the enzymic cyclization reactions are proposed. © 1977. | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Archives of Biochemistry and Biophysics | |
dc.subject | Biophysics | |
dc.subject | Biochemistry | |
dc.subject | Molecular Biology | |
dc.title | Biosynthesis of monoterpene hydrocarbons from [1-3H]neryl pyrophosphate and [1-3H]geranyl pyrophosphate by soluble enzymes from Citrus limonum | |
dc.type | Artículo de revista | |