Artículos de revistas
Evaluation Of Hypoxia Inducible Factor Targeting Pharmacological Drugs As Antileishmanial Agents
Registro en:
Asian Pacific Journal Of Tropical Medicine. Elsevier Sci Ltd, v. 9, p. 633 - 638, 2016.
1995-7645
WOS:000378577500006
10.1016/j.apjtm.2016.05.018
Autor
Pelegrini
Marina Dal'Bo; Pereira
Juliana Biar; Costa
Solange dos Santos; Salazar Terreros
Myriam Janeth; Degrossoli
Adriana; Giorgio
Selma
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) To evaluate whether hypoxia inducible factor (HIF-1 alpha) targeting pharmacological drugs, echinomycin, resveratrol and CdCl2, which inhibit HIF-1 alpha stimulation, and mimosine, which enhances the stability of HIF-1 alpha present antileishmanial properties. Methods: The leishmanicidal effect of drugs was evaluated in mouse macrophages and Balb/c mouse model for cutaneous leishmaniosis. Results: Resveratrol and CdCl2, reduced the parasite load [IC50, (27.3 +/- 2.25) mu M and (24.8 +/- 0.95) mu M, respectively]. The IC50 value of echinomycin was (22.7 +/- 7.36) mu M and mimosine did not alter the parasite load in primary macrophages. The macrophage viability IC50 values for resveratrol, echinomycin and CdCl2, and mimosine were >40 mu M, >100 mu M > 200 mu M and>2 000 mu M, respectively. In vivo no differences between cutaneous lesions from control, resveratrol- and echinomycin-treated Balb/c mice were detected. Conclusions: Resveratrol, echinomycin and CdCl2, reduce parasite survival in vitro. The HIF-1 alpha targeting pharmacological drugs require further study to more fully determine their anti-Leishmania potential and their role in therapeutic strategies. 9 7 633 638 Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil [2009/10771-9] Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil [301052/2009-3] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)