Artículos de revistas
Knocking Down Amygdalar Ptp1b In Diet-induced Obese Rats Improves Insulin Signaling/action, Decreases Adiposity And May Alter Anxiety Behavior
Registro en:
Metabolism-clinical And Experimental. W B Saunders Co-elsevier Inc, v. 70, p. 1 - 11, 2017.
0026-0495
1532-8600
WOS:000400037600001
10.1016/j.metabol.2017.01.029
Autor
Mendes
Natalia Ferreira; Castro
Gisele; Guadagnini
Dioze; Tobar
Natalia; Cognuck
Susana Quiros; Kagohara Elias
Lucila Leico; Boer
Patricia Aline; Prada
Patricia Oliveira
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Protein tyrosine phosphatase 1B (PTP1B) has been extensively implicated in the regulation of body weight, food intake, and energy. expenditure. The role of PTP1B appears to be cell and brain region dependent. Results. Herein, we demonstrated that chronic high-fat feeding enhanced PTP1B expression in the central nucleus of the amygdala (CeA) of rats compared to rats on chow: Knocking down PTP1B with oligonucleotide antisense (ASO) decreased its expression and was sufficient to improve the anorexigenic effect of insulin through IR/Akt signaling in the CeA. ASO treatment reduces body weight, fat mass, serum leptin levels, and food intake and also increases energy expenditure, without altering ambulatory activity. These changes were explained, at least in part, by the improvement of insulin sensitivity in the CeA, decreasing NPY and enhancing oxytocin expression. There was a slight decline in fasting blood glucose and serum insulin levels possibly due to leanness in rats treated with ASO. Surprisingly, the elevated plus maze test revealed an anxiolytic behavior after reduction of PTP1B in the CeA. Conclusions. Thus, the present study highlights the deleterious role that the amygdalar PTP1B has on energy homeostasis in obesity states. The reduction of PTP1B in the CeA may be a strategy for the treatment of obesity, insulin resistance and anxiety disorders. (C) 2017 Elsevier Inc. All rights reserved. 70 1 11 FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo): Auxflio Regular [2015/00343-0] CEPID, Sao Paulo, Brazil [2013/07607-8] CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) Universal [481, 084/2013-4] INCT (Instituto Nacional Ciencia e Tecnologia de Obesidade e Diabetes) [573, 856/2008-7] CAPES fellowship, Brasilia, Brazil Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)