Artículos de revistas
Hypoxia, hypoxia-inducible factor-1 alpha and vascular endothelial growth factor in a murine model of Schistosoma mansoni infection
Experimental And Molecular Pathology. Academic Press Inc Elsevier Science, v. 89, n. 3, n. 327, n. 333, 2010.
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Schistosomiasis mansoni is a chronic parasitic disease where much of the symptomatology is attributed to granuloma formation, an immunopathological reaction against Schistosoma eggs. To more clearly understand the immunopathology of schistosomiasis, the tissue microenvironment generated by S. marisoni infected mice was investigated. Using the hypoxia marker pimonidazole, we provide immunohistochemical evidence that hypoxia occurred in inflammatory cells infiltrated around the eggs and cells surrounding granulomas in the liver, intestine, spleen and lungs of infected mice. Hypoxia-inducible factor-let (HIF-1 alpha) was mainly expressed in inflammatory cells surrounding the eggs and in hepatocytes surrounding cellular and fibrocellular granulomas in infected mouse liver. HIF-1 alpha expression was also verified in granulomas in the other tissues tested (intestine, spleen and lungs). Vascular endothelial growth factor (VEGF) expression was observed in the extracellular space surrounding inflammatory cells in liver granuloma. The VEGF expression pattern verified in infected mouse liver was very similar to that observed in the other tissues tested. A strong positive correlation occurred between pimonidazole binding and HIF-1 alpha and VEGF expression in the tissues tested, except for lung. This work is the first evidence that infection by a helminth parasite, S. mansoni, produces a hypoxic tissue microenvironment and induces HIF-1 alpha and VEGF expression. (C) 2010 Elsevier Inc. All rights reserved.893327333Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Showing items related by title, author, creator and subject.
Fermentative enzymes activity in soybean roots and nodules under hypoxia and post-hypoxia conditions. BORELLA, J.; DURIGON, M. A.; AMARANTE, L. do.; EMYGDIO, B. M.
Hypoxia and temperature: does hypoxia affect caiman embryo differentiation rate or rate of growth only? Iungman, Josefina Luciana; Piña, Carlos Ignacio (Elsevier, 2013-05)In crocodilians, the rate of embryonic development and consequently many posthatch attributes are affected by temperature. Since temperature exhibits strong influences on fitness (embryo survivorship and phenotype) by ...
Gene expression promoted by the SV40 DNA targeting sequence and the hypoxia-responsive element under normoxia and hypoxia Sacramento, Chester Bittencourt; Moraes, Jane Zveiter de; Denapolis, P.m.a.; Han, Sang Won (Associação Brasileira de Divulgação Científica, 2010-08-01)The main objective of the present study was to find suitable DNA-targeting sequences (DTS) for the construction of plasmid vectors to be used to treat ischemic diseases. The well-known Simian virus 40 nuclear DTS (SV40-DTS) ...