Hypoxia, hypoxia-inducible factor-1 alpha and vascular endothelial growth factor in a murine model of Schistosoma mansoni infection

dc.creatorAraujo, AP
dc.creatorFrezza, TF
dc.creatorAllegretti, SM
dc.creatorGiorgio, S
dc.date2010
dc.dateDEC
dc.date2014-11-15T02:58:10Z
dc.date2015-11-26T16:30:30Z
dc.date2014-11-15T02:58:10Z
dc.date2015-11-26T16:30:30Z
dc.date.accessioned2018-03-28T23:11:33Z
dc.date.available2018-03-28T23:11:33Z
dc.identifierExperimental And Molecular Pathology. Academic Press Inc Elsevier Science, v. 89, n. 3, n. 327, n. 333, 2010.
dc.identifier0014-4800
dc.identifierWOS:000284683300018
dc.identifier10.1016/j.yexmp.2010.09.003
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69092
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69092
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69092
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1269975
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionSchistosomiasis mansoni is a chronic parasitic disease where much of the symptomatology is attributed to granuloma formation, an immunopathological reaction against Schistosoma eggs. To more clearly understand the immunopathology of schistosomiasis, the tissue microenvironment generated by S. marisoni infected mice was investigated. Using the hypoxia marker pimonidazole, we provide immunohistochemical evidence that hypoxia occurred in inflammatory cells infiltrated around the eggs and cells surrounding granulomas in the liver, intestine, spleen and lungs of infected mice. Hypoxia-inducible factor-let (HIF-1 alpha) was mainly expressed in inflammatory cells surrounding the eggs and in hepatocytes surrounding cellular and fibrocellular granulomas in infected mouse liver. HIF-1 alpha expression was also verified in granulomas in the other tissues tested (intestine, spleen and lungs). Vascular endothelial growth factor (VEGF) expression was observed in the extracellular space surrounding inflammatory cells in liver granuloma. The VEGF expression pattern verified in infected mouse liver was very similar to that observed in the other tissues tested. A strong positive correlation occurred between pimonidazole binding and HIF-1 alpha and VEGF expression in the tissues tested, except for lung. This work is the first evidence that infection by a helminth parasite, S. mansoni, produces a hypoxic tissue microenvironment and induces HIF-1 alpha and VEGF expression. (C) 2010 Elsevier Inc. All rights reserved.
dc.description89
dc.description3
dc.description327
dc.description333
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationExperimental And Molecular Pathology
dc.relationExp. Mol. Pathol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectSchistosoma mansoni
dc.subjectGranuloma
dc.subjectHypoxia
dc.subjectAngiogenesis
dc.subjectMice
dc.subjectTumor Hypoxia
dc.subjectLeishmania-amazonensis
dc.subjectInflammatory Cells
dc.subjectExpression
dc.subjectOxygen
dc.subjectAngiogenesis
dc.subjectHif-1-alpha
dc.subjectHypoxia-inducible-factor-1-alpha
dc.subjectGranulomas
dc.subjectMice
dc.titleHypoxia, hypoxia-inducible factor-1 alpha and vascular endothelial growth factor in a murine model of Schistosoma mansoni infection
dc.typeArtículos de revistas


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