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Mostrando ítems 11-20 de 201
GFP transient expression and silencing in fragaria x ananassa
(IOS Press, 2020-01)
Background: Stable transformation, transient expression, and post-transcriptional gene silencing (PTGS) are powerful methodologies that allow exploration of gene function. Objective: We aimed to apply these methodologies ...
An apomixis-linked ORC3-like pseudogene is associated with silencing of its functional homolog in apomictic Paspalum simplex
(Oxford University Press, 2016-03)
Apomixis in plants consists of asexual reproduction by seeds. Here we characterized at structural and functional levels an apomixis-linked sequence of Paspalum simplex homologous to subunit 3 of the ORIGIN RECOGNITION ...
Becoming a Selfish Clan: Recombination Associated to Reverse-Transcription in LTR Retrotransposons
(Oxford University Press, 2019-11)
Transposable elements (TEs) are parasitic DNA bits capable of mobilization and mutagenesis, typically suppressed by host's epigenetic silencing. Since the selfish DNA concept, it is appreciated that genomes are also molded ...
TMV induces RNA decay pathways to modulate gene silencing and disease symptoms
(Wiley Blackwell Publishing, Inc, 2017-01)
RNA decay pathways comprise a combination of RNA degradation mechanisms that are implicated in gene expression, development and defense responses in eukaryotes. These mechanisms are known as the RNA Quality Control or RQC ...
A Comprehensive Haplotype Targeting Strategy for Allele-Specific HTT Suppression in Huntington Disease.
(Elsevier, 2019)
Huntington disease (HD) is a fatal neurodegenerative disorder caused by a gain-of-function mutation in HTT. Suppression of mutant HTT has emerged as a leading therapeutic strategy for HD, with allele-selective approaches ...
TMV induces RNA decay pathways to modulate gene silencing and disease symptoms
(2017)
RNA decay pathways comprise a combination of RNA degradation mechanisms that are implicated in gene expression, development and defense responses in eukaryotes. These mechanisms are known as the RNA Quality Control or RQC ...
Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
(Nature Publishing Group, 2009-10)
The sunflower belongs to the Compositae family and is an economically important crop because of the quality of its oil. Unfortunately, molecular analyses are limited due to the lack of genomic information, mutant libraries ...
Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
(Springer, 2013-02)
Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione ...
Silencing of two vitellogenin genes inhibits oviposition in the chagas disease vector triatoma infestans (Hemiptera: Reduviidae)
(American Society of Tropical Medicine and Hygiene, 2017-05-30)
RNA interference caused by exogenous double-stranded RNA (dsRNA) is used to downregulate crucial genes to control insects. The reproductive success of all oviparous species depends on vitellogenin (Vg) biosynthesis and its ...
Coupling virus-induced gene silencing to exogenous green fluorescence protein expression provides a highly efficient system for functional genomics in arabidopsis and across all stages of tomato fruit development
(2011-07)
Since the advent of the postgenomic era, efforts have focused on the development of rapid strategies for annotating plant genes of unknown function. Given its simplicity and rapidity, virus-induced gene silencing (VIGS) ...