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Novel biotechnological platform based on baculovirus occlusion bodies carrying Babesia bovis small antigenic peptides for the design of a diagnostic enzyme-linked immunosorbent assay (ELISA)
(Springer, 2018-01)
Baculoviruses are large DNA virus of insects principally employed in recombinant protein expression. Its ability to form occlusion bodies (OBs), which are composed mainly of polyhedrin protein (POLH), makes them ...
Expression of human proacrosin in Escherichia coli and binding to zona pellucida
(Society for the Study of Reproduction, 2000)
Proacrosin is a multifunctional protein present in the sperm acrosome. This study characterizes the expression of human proacrosin in bacteria and assesses zona pellucida binding activity. The cDNA encoding human proacrosin ...
In situ removal of consensus dengue virus envelope protein domain III fused to hydrophobin in Pichia pastoris cultures
(Academic Press Inc Elsevier Science, 2019-01)
This work describes a novel strategy for the integrated expression and purification of recombinant proteins in Pichia pastoris cultures. Hydrophobins can be used as fusion tags, proteins fused to them alter their hydrophobicity ...
Generation of affinity-tagged fluoromycobacteriophages by mixed assembly of phage capsids
(American Society for Microbiology, 2013-09)
Addition of affinity tags to bacteriophage particles facilitates a variety of applications, including vaccine construction and diagnosis of bacterial infections. Addition of tags to phage capsids is desirable, as modification ...
cDNA cloning, heterologous expression, protein folding and immunogenic properties of a phospholipase A2 from Bothrops ammodytoides venom
(Academic Press Inc Elsevier Science, 2019-02)
A mRNA transcript that codes for a phospholipase (PLA2) was isolated from a single venom gland of the Bothrops ammodytoides viper. The PLA2 transcript was cloned onto a pCR®2.1-TOPO vector and subsequently expressed ...
Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
(Elsevier Science, 2009-07)
Our previous studies reported the inhibitory action against arenaviruses of antiretroviral zinc finger-reactive compounds provided by the National Cancer Institute (USA). These compounds were able to inactivate virions as ...
Evaluation of testicular sperm CRISP2 as a potential target for contraception
(American Society of Andrology, 2012-11-12)
Cysteine-rich secretory protein 2 (CRISP2) is a testicular sperm protein proposed to be involved in fertilization. With the aim of examining the relevance of CRISP2 for fertility and its potential use as a target for ...
Discovery of a Potent and Selective Chikungunya Virus Envelope Protein Inhibitor through Computer-Aided Drug Design
(American Chemical Society Inc, 2021-05)
The worldwide expansion of chikungunya virus (CHIKV) into tropical and subtropical areas in the last 15 years has posed a currently unmet need for vaccines and therapeutics. The E2-E1 envelope glycoprotein complex binds ...