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In vitro and in vivo inhibition of Hass avocado polyphenol oxidase enzymatic browning by paeonol, beta-cyclodextrin, and paeonol:beta-cyclodextrin inclusion complex
(Soc Bioscience Bioengineering Japan, 2019-06)
Polyphenol oxidase (PPO) was extracted from Hass avocados and its physicochemical properties were analyzed. The optimum pH and temperature of the enzyme were pH 7.5 and 20°C. This PPO showed a high thermal stability, since ...
Radio-synthesized protein-based nanoparticles for biomedical purposes
(Pergamon-Elsevier Science Ltd, 2014-01)
Protein-crosslinking whether done by enzymatic or chemically induced pathways increases the overall stability of proteins. In the continuous search for alternative routes for protein stabilization we report a novel technique ...
Evidence of structural changes of an enzymatic extract entrapped into alginate beads
(Elsevier Science Sa, 2013-01)
In this work, we analyzed the structural changes of araujiain entrapped into alginate beads. Araujiain is an enzymatic preparation containing three known enzymatic fractions with each fraction individually presenting a ...
Stability of oil: Water emulsions of amaranth proteins: Effect of hydrolysis and pH
(Elsevier, 2010-08)
In this contribution we have determined the effect of limited enzymatic hydrolysis on the emulsifying capacity of amaranth proteins. The action of enzyme (alcalase and trypsin) and the pH of the continuous phase of the ...
Seaweed-based natural ingredients: Stability of phlorotannins during extraction, storage, passage through the gastrointestinal tract and potential incorporation into functional foods
(Elsevier Science, 2020-11)
Adding value to seaweed by extracting their different bioactive compounds and incorporating them into foods represent an interesting and strategic approach to diversify the functional foods offer. However, once harvested, ...
Structure of the Human ACP-ISD11 Heterodimer
(American Chemical Society, 2019-10)
In recent years, the mammalian mitochondrial protein complex for iron-sulfur cluster assembly has been the focus of important studies. This is partly because of its high degree of relevance in cell metabolism and because ...
Enzyme-Free Immunoassay Using Silver Nanoparticles for Detection of Gliadin at Ultralow Concentrations
(American Chemical Society, 2018-02)
Determination of biomarkers in clinical or food samples is of crucial importance for monitoring, prevention, and care of public health. The standard procedure used for this purpose is the enzyme-linked immunosorbent assay ...
Fabry disease: Treatment and diagnosis
(John Wiley & Sons Inc, 2009-11)
Fabry disease is an X-linked lysosomal disorder that results from a deficiency of the lysosomal enzyme α-galactosidase A leading to accumulation of glycolipids, mainly globotriaosylceramide in the cells from different ...
Biodegradation of vegetable residues by polygalacturonase-agar using a trickle-bed bioreactor
(Institution of Chemical Engineers, 2018-09)
Bacterial pectinases degrade the pectic substances present in plant tissues and particularly, polygalacturonases catalyze the hydrolysis of α-(1,4) glycosidic bonds linking D-galacturonic acid units. In this study, ...