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Analytical and Clinical Validation for RT-qPCR Detection of SARS-CoV-2 Without RNA Extraction
(Frontiers Media, 2020)
Background: The recent COVID-19 pandemic has posed an unprecedented challenge to laboratory diagnosis, based on the amplification of SARS-CoV-2 RNA. With global contagion figures exceeding 4 million persons, the shortage ...
Evaluation of Nifurtimox Treatment of Chronic Chagas Disease by Means of Several Parasitological Methods
(American Society for Microbiology, 2013-09)
Currently, evaluation of drug efficacy for Chagas disease remains a controversial issue with no consensus. In this work, we evaluated
the parasitological efficacy of Nifurtimox treatment in 21 women with chronic Chagas ...
Discrete typing units of Trypanosoma cruzi detected by real-time PCR in Chilean patients with chronic Chagas cardiomyopathy
(Elsevier B.V., 2018)
© 2018 Elsevier B.V. Chagas disease is a major public health problem in Latin America and has spread to other countries due to immigration of infected persons. 10–30% of patients with chronic Chagas disease will develop ...
Acemannan and fructans from Aloe vera (Aloe barbadensis Miller) plants as novel prebiotics
(American Chemical Society, 2017)
The nutraceutical properties of Aloe vera have been attributed to a glucomannan known as acemannan. Recently information has been published about the presence of fructans in Aloe vera but there are no publications about ...
Caracterización molecular del Potato virus V (PVV) infectando Solanum phureja mediante secuenciación de nueva generación
(Universidad Nacional de Colombia - Sede Bogotá - Faculdad de Ciencias - Departamento de Biología, 2016-09-01)
Las enfermedades virales son uno de los problemas más limitantes para la producción de papa en el mundo. Uno de los materiales de papa más susceptibles a los virus corresponde a Solanum phureja; sin embargo, en Colombia ...
Transcriptome sequencing of Prunus sp. rootstocks roots to identify candidate genes involved in the response to root hypoxia
(Springer, 2015)
Root hypoxia in fruit trees affects growth, vegetative
development, and reproductive development, which
is reflected in low productivity, poor fruit quality, and
premature decay of trees. Using Illumina Hiseq2000, we ...
RNA-Seq analysis and transcriptome assembly of raspberry fruit (Rubus idaeus ¨Heritage¨) revealed several candidate genes involved in fruit development and ripening
(Elsevier B.V., 2019)
Using Illumina HiSeq ™ 2000 sequencing platform (100 bp double-end reads), we performed transcriptome analysis of flower (F), green (G) and pink (P) fruit stages of red raspberry. Transcriptome was obtained by the de-novo ...
Expression of two indole-3-acetic acid (IAA)-amido synthetase (GH3) genes during fruit development of raspberry (Rubus idaeus Heritage)
(Elsevier, 2019)
The conjugation of indole-3-acetic acid (IAA) to amino acids by indole-3-acetic acid (IAA)-amido synthetases (GH3) is an important part of auxin level regulation. However, the auxin conjugation during development of soft ...
Chilean IPNV isolates: Robustness analysis of PCR detection
(Universidad Católica de Valparaíso, 2016)
Background: The genomes of several infectious pancreatic necrosis viruses (IPNVs) isolated in Chile were sequenced with a single amplification approach for both segments A and B. The resulting sequences were then used to ...
Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
(Elsevier, 2015)
An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and ...