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Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
(2009-04-01)
Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A ...
Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation
(1999-02-01)
Hrp1p is a heterogeneous ribonucleoprotein (hnRNP) from the yeast Saccharomyces cerevisiae that is involved in the cleavage and polyadenylation of the 3'-end of mRNAs and mRNA export. In addition, Hrp1p is one of several ...
Melatonin and testicular function: Characterization of binding sites for 2-[125I]-iodomelatonin in immature rat testes
(Elsevier Inc., 1997)
Melatonin-binding sites in membrane preparation of immature rat testes were demonstrated by utilizing 2-[125I]-iodomelatonin as a radioligand. Binding at these sites was found to be reversible, saturable, specific and of, ...
Probing the binding of the coumarin drugs using peptide fragments of DNA gyrase B protein
(2005-05-01)
Bacterial DNA gyrase, has been identified as the target of several antibacterial agents, including the coumarin drugs. The coumarins inhibit the gyrase action by competitive binding to the ATP-binding site of DNA gyrase B ...
Identification of a New Hormone-Binding Site on the Surface of Thyroid Hormone Receptor
(Endocrine SocWashingtonEUA, 2014)
CHARACTERISTICS OF ZINC-BINDING TO HUMAN RED-BLOOD-CELL MEMBRANES
(Wiley-Blackwell, 1994-01-01)
The objective of the present study was to standardize the analysis of zinc binding on human red blood cell (RBC) membranes in 20 normal adults. The displacement studies revealed that at the maximal stable zinc concentration ...
Binding of fluorescent dansyl amino acids in albumin: When access to the protein cavity is more important than the strength of binding
(2021-04-01)
Human serum albumin (HSA) is the primary drug carrier protein in the blood plasma. To perform this task, it has two cavities (sites I and II) where the pharmaceutical drugs can be bound. Dansyl amino acids are fluorescent ...
Albumin-induced circular dichroism in Congo red: applications for studies of amyloid-like fibril aggregates and binding sites
(Elsevier B. V., 2015-11-05)
Congo red (CR), one of the most commonly used dyes for the identification of amyloid fibril aggregates, is also a ligand of native bovine serum albumin (BSA). Induced circular dichroism (ICD) is a phenomenon observed when ...
Probing the Specificity of Binding to the Major Nuclear Localization Sequence-binding Site of Importin-alpha Using Oriented Peptide Library Screening
(Amer Soc Biochemistry Molecular Biology Inc, 2010-06-25)
Importin-alpha is the nuclear import receptor that recognizes the classic monopartite and bipartite nuclear localization sequences (cNLSs), which contain one or two clusters of basic amino acids, respectively. Different ...