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Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies
(Elsevier B.V., 2002-12-23)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to ...
Thermostable conidial and mycelial alkaline phosphatases from the thermophilic fungus Scytalidium thermophilum
(Nature America Inc, 2001-10-01)
An extracellular (conidial) and an intracellular (mycelial) alkaline phosphatase from the thermophilic fungus Scytalidium thermophilum were purified by DEAE-cellulose and Concanavalin A-Sepharose chromatography. These ...
Culture of osteogenic cells from human alveolar bone: A useful source of alkaline phosphatase
(Elsevier B.V., 2007-11-01)
The aim of this study was to obtain membrane-bound alkaline phosphatase from osteoblastic-like cells of human alveolar bone. Cells were obtained by enzymatic digestion and maintained in primary culture in osteogenic medium ...
Triton X-100 solubilized bone matrix-induced alkaline phosphatase
(1987-12-01)
1. 1. Solubilized and membrane-bound alkaline phosphatase showed Michaelis-Menten behavior in a wide range of different substrate concentrations. 2. 2. Membrane-bound alkaline phosphatase has a molecular weight of 130,000 ...
THE PHO-2A MUTANT OF NEUROSPORA-CRASSA WHICH IS DEFICIENT IN PI-REPRESSIBLE ALKALINE-PHOSPHATASE (EC 3.1.3.1) IS ALSO DEFECTIVE IN PI-REPRESSIBLE ACID-PHOSPHATASE (EC 3.1.3.2)
(Associação Brasileira de Divulgação Científica (ABRADIC), 1992-01-01)
1. The mycelial Pi-repressible acid phosphatase presented p-nitrophenylphosphatase activity with negative cooperativity and Michaelian behavior when synthesized by the wild-type and pho-2A mutant strains of Neurospora ...
The dermatophyte Trichophyton rubrum secretes an EDTA-sensitive alkaline phosphatase on high-phosphate medium
(2003-12-01)
In this communication, we show that the growth of isolate H6 of the dermatophyte Trichophyton rubrum on non-buffered medium and under saturating phosphate conditions is dependent on the initial growth pH, with an apparent ...
Construction of an alkaline phosphatase-liposome system: a tool for biomineralization study
(Elsevier B.V., 2002-09-01)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper. we standardize a method for ...
Allosteric modulation of pyrophosphatase activity of rat osseous plate alkaline phosphatase by magnesium ions
(Elsevier B.V., 1998-01-01)
Pyrophosphatase activity of rat osseous plate alkaline phosphatase was studied at different concentrations of calcium and magnesium ions. with the aim of characterizing the modulation of enzyme activity by these metals. ...
Inorganic pyrophosphate-phosphohydrolytic activity associated with rat osseous plate alkaline phosphatase
(Cellular & Molecular Biology, 1998-03-01)
Purified membrane-bound alkaline phosphatase from rat osseous plate hydrolyzed pyrophosphate in the presence of magnesium ions, with a specific activity of 92.7 U/mg. Optimal apparent pH for pyrophosphatase activity was ...