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Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies
(Elsevier B.V., 2002-12-23)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to ...
Thermostable conidial and mycelial alkaline phosphatases from the thermophilic fungus Scytalidium thermophilum
(Nature America Inc, 2001-10-01)
An extracellular (conidial) and an intracellular (mycelial) alkaline phosphatase from the thermophilic fungus Scytalidium thermophilum were purified by DEAE-cellulose and Concanavalin A-Sepharose chromatography. These ...
Purification and characterization of an iron-activated alkaline phosphatase produced by rhizopus microsporus var. Microsporus under submerged fermentation using rye flour
(2020-07-01)
Current researches have been carried out to find microorganisms that can produce enzymes for different biotechnological purposes. Among the enzymes, the microbial phosphatases, responsible for hydrolyzing phosphoric acid ...
Construction of an alkaline phosphatase-liposome system: a tool for biomineralization study
(Elsevier B.V., 2002-09-01)
Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper. we standardize a method for ...
Alkaline phosphatase activity in salivary gland cells of Rhodnius neglectus and R-prolixus (Hemiptera, Triatominae)
(Funpec-editora, 2016-01-01)
Alkaline phosphatase activity was detected in salivary gland cells of the Rhodnius neglectus Lent, 1954, and R. prolixus Stal, 1859, vectors of Trypanosoma cruzi Chagas, 1909 (etiological agent of Chagas disease) and T. ...
Culture of osteogenic cells from human alveolar bone: A useful source of alkaline phosphatase
(Elsevier B.V., 2007-11-01)
The aim of this study was to obtain membrane-bound alkaline phosphatase from osteoblastic-like cells of human alveolar bone. Cells were obtained by enzymatic digestion and maintained in primary culture in osteogenic medium ...
Conidial alkaline phosphatase from Neurospora crassa
(1996-01-01)
An alkaline phosphatase was purified from conidia of a Neurospora crassa wild type strain. The Mr of the purified native enzyme was estimated as ca 145000 and 110000 by gel filtration, in the presence and absence of magnesium ...
Triton X-100 solubilized bone matrix-induced alkaline phosphatase
(1987-12-01)
1. 1. Solubilized and membrane-bound alkaline phosphatase showed Michaelis-Menten behavior in a wide range of different substrate concentrations. 2. 2. Membrane-bound alkaline phosphatase has a molecular weight of 130,000 ...
OSSEOUS PLATE ALKALINE-PHOSPHATASE IS ANCHORED BY GPI
(Associação Brasileira de Divulgação Científica (ABRADIC), 1994-02-01)
Alkaline phosphatase activity was released up to 100% from the membrane by using 0.1 U of phosphatidylinositol-specific phospholipase C from B. thuringiensis. The Mr of solubilized enzyme was 145,000 by Sephacryl S-300 gel ...