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Fluorescence resonance energy transfer and molecular modeling studies on 4 ',6-diamidino-2-phenylindole (DAPI) complexes with tubulin
(COLD SPRING HARBOR LAB PRESS, 2006-03)
The goal of this work was to determine the binding properties and location of 4',6-diamidino-2-phenyl=indole (DAPI) complexed with tubulin. Using fluorescence anisotropy, a dissociation constant of 5.2 +/- 0.4 mu M for the ...
Tubulin polyglutamylation is a general traffic-control mechanism in hippocampal neurons
(Company of Biologists, 2020)
Neurons are highly complex cells that heavily rely on intracellular transport to distribute a range of functionally essential cargoes within the cell. Post-translational modifications of tubulin are emerging as mechanisms ...
Carboxyl terminal sequences of β-tubulin involved in the interaction of HMW-MAPs. Studies using site-specific antibodies
(Kluwer Academic Publishers, 1994)
After the finding of the involvement of the C-terminal moieties of tubulin subunits in the interaction of MAPs, different studies have focused on the substructure of the binding domains for the different MAPs. Current ...
Aminochrome Toxicity is Mediated by Inhibition of Microtubules Polymerization Through the Formation of Adducts with Tubulin
(Springer New York LLC, 2016)
© 2015, Springer Science+Business Media New York.In this study, we investigated the role of adducts formation between aminochrome and tubulin and its interference in microtubules assembly and stability in aminochrome-induced ...
Helicity of α(404-451) and β(394-445) tubulin C-terminal recombinant peptides
(1999)
We have investigated the solution conformation of the functionally relevant C-terminal extremes of α- and β-tubulin, employing the model recombinant peptides RL52α3 and RL33β6, which correspond to the amino acid sequences ...
Tubulin and microtubule‐associated protein pools in unfertilized and fertilized eggs of the trout Oncorhynchus mykiss
(1995)
A molecular characterization of tubulin and microtubule‐associated proteins (MAPs) along with their intracellular pool distributions in both unfertilized and fertilized oocytes of the trout Oncorhynchus mykiss was carried ...
The Binding of terbium Ions to tubulin induces ring formation
(1993)
The intrinsic fluorescence excitation and emission spectra of chicken brain tubulin showed the characteristic tryptophan fluorescence. The emission spectrum of Tb3+ in the presence of tubulin and GTP excited at 295 nm, ...
Tubulin, actin, and tau protein interactions and the study of their macromolecular assemblies
(2002)
The intracellular polymerization of cytoskeletal proteins into their supramolecular assemblies raises many questions regarding the regulatory patterns that control this process. Binding experiments using the ELISA solid ...
The chaperonin CCT promotes the formation of fibrillar aggregates of gamma-tubulin
(Elsevier, 2018)
The type II chaperonin CCT is involved in the prevention of the pathogenesis of numerous human misfolding disorders, as it sequesters misfolded proteins, blocks their aggregation and helps them to achieve their native ...
Microtubule-associated protein 1B interaction with tubulin tyrosine ligase contributes to the control of microtubule tyrosination
(2007)
Microtubule-associated protein 1B (MAP1B) is the first microtubule- associated protein to be expressed during nervous system development. MAP1B belongs to a large family of proteins that contribute to the stabilization ...