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Activity-Structure Study on the Peptide Fraction of AG2: a Potent In Vitro Transfection Agent
(Humana Press, 2019-05)
Gemini-based amphiphiles are candidates for biomedical applications. In fact, most of thegemini compounds described in the literature have been prepared to be used as new syntheticvectors in gene transfection. Our group ...
Transfection of bovine fetal fibroblast with polyethylenimine (PEI) nanoparticles: effect of particle size and presence of fetal bovine serum on transgene delivery and cytotoxicity
(Springer, 2017-08)
The development of efficient transfection protocols for livestock cells is crucial for implementation of cell-based transgenic methods to produce genetically modified animals. We synthetized fully deacylated linear 22, 87 ...
Transfection of Capsaspora owczarzaki, a close unicellular relative of animals
(Company of Biologists, 2018-05)
How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal ...
Improved synthesis and characterization of cholesteryl oleate-loaded cationic solid lipid nanoparticles with high transfection efficiency for gene therapy applications
(Elsevier Science, 2019-08)
The development of new nanoparticle formulations that are capable of high transfection efficiency without toxicity is essential to provide new tools for gene therapy. However, the issues of complex, poorly reproducible ...
Development and characterization of an improved formulation of cholesteryl oleate-loaded cationic solid-lipid nanoparticles as an efficient non-viral gene delivery system
(Elsevier Science, 2019-09)
Nanoparticle-mediated plasmid delivery is considered a useful tool to introduce foreign DNA into the cells for the purpose of DNA vaccination and/or gene therapy. Cationic solid-lipid nanoparticles (cSLNs) are considered ...
DOTAP, a lipidic transfection reagent, triggers Arabidopsis plant defense responses
(Springer, 2019-02)
Main conclusion: DOTAP triggers Arabidopsis thaliana immunity and by priming the defense response is able to reduce bacterial pathogen attack. DOTAP is a cationic lipid widely used as a liposomal transfection reagent and ...
Disruption of the D2 dopamine receptor alters GH and IGF-I secretion and causes dwarfism in male mice
(Endocrine Society, 2002-04)
We determined the consequences of the loss of D2 receptors (D2R) on the GH-IGF-I axis using mice deficient in functional dopamine D2 receptors by targeted mutagenesis (D2R(-/-)). Body weights were similar at birth, but ...
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
(PLOS, 2013-08-20)
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). ...
Enhancement of nucleic acid delivery to hard-to-transfect human colorectal cancer cells by magnetofection at laminin coated substrates and promotion of the endosomal/lysosomal escape
(Royal Society of Chemistry, 2015)
Despite a great diversity of nanomaterials, such as cationic lipid, polymers or inorganic nanoparticles, has been developed in order to carry nucleic acids across plasma membrane, these methodologies have still insufficient ...
Producción de partículas virales pseudotipadas SARS-CoV-2 para la evaluación de nuevas terapias para COVID-19Production of SARS-CoV-2 pseudotyped viral particles for the evaluation of new therapies against COVID-19
(Universidad Nacional del Litoral, 2022-05)
La inmunoterapia pasiva como tratamiento contra el virus SARS-CoV-2 (del inglés, severe acute respiratory syndrome coronavirus-2) requiere de la cuantificación de la capacidad neutralizante de los anticuerpos para el ...