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Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer
(Cambridge University Press, 2005-11-01)
As an important step in the nuclear transfer (NT) procedure, we evaluated the effect of three different treatments for oocyte activation on the in vitro and in vivo developmental capacity of bovine reconstructed embryos: ...
Aberrant expression of E-cadherin and beta-catenin proteins in placenta of bovine embryos derived from somatic cell nuclear transfer
(CSIRO Publishing, 2012-01-01)
Abnormal placental development is common in the bovine somatic cell nuclear transfer (SCNT)-derived fetus. In the present study, we characterised the expression of E-cadherin and beta-catenin, structural proteins of adherens ...
Compounds used to produce cloned animals are genotoxic and mutagenic in mammalian assays in vitro and in vivo
(Associação Brasileira de Divulgação Científica (ABRADIC), 2014)
Somatic cell nuclear transfer is associated with altered expression of angiogenic factor systems in bovine placentomes at term
(Funpec-editora, 2010-01-01)
Low efficiency of somatic cell cloning by nuclear transfer has been associated with alterations of placental vascular architecture. Placental growth and function depend on the growth of blood vessels; VEGF-A and bFGF are ...
Chemically Assisted Enucleation Results in Higher G6PD Expression in Early Bovine Female Embryos Obtained by Somatic Cell Nuclear Transfer
(Mary Ann Liebert, Inc., 2012-10-01)
Despite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would ...
Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring
(Mary Ann Liebert, Inc., 2010-06-01)
Nuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer ...
Influência do fator de crescimento semelhante à insulina I (IGF-I) adicionado ao meio fluido sintético de tuba uterina (SOF) sobre a maturação in vitro de oócitos caninos (Canis familiaris)
(Universidade Estadual de Londrina (UEL), 2007-07-01)
The follicular growth and oocyte maturation knowledge are very important to the development and improvement of new biotechnologies such as in vitro fertilization and somatic cell nuclear transfer. In order to the necessity ...
Macroscopic and Microscopic findings in a set of congenital anomalies in two calves produced through in Vitro production
(2013-08-22)
In vitro production and somatic cell nuclear transfer are biotechnologies widely used for breeding cattle, although may result in congenital anomalies. This paper aims to report a set of congenital anomalies in two Nelore ...