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Exploring dynamics in living cells by tracking single particles
(Springer, 2007-05-25)
In the last years, significant advances in microscopy techniques and the introduction of a novel technology to label living cells with genetically encoded fluorescent proteins revolutionized the field of Cell Biology. Our ...
Quantifying transcription factor binding dynamics at the single-molecule level in live cells
(Academic Press Inc Elsevier Science, 2017-07)
Progressive, technological achievements in the quantitative fluorescence microscopy field are allowing researches from many different areas to start unraveling the dynamic intricacies of biological processes inside living ...
Single-molecule analysis of steroid receptor and cofactor action in living cells
(Nature Publishing Group, 2017-06-21)
Population-based assays have been employed extensively to investigate the interactions of transcription factors (TFs) with chromatin and are often interpreted in terms of static and sequential binding. However, fluorescence ...
Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
(Cell Press, 2019-09-19)
Genes are transcribed in a discontinuous pattern referred to as RNA bursting, but the mechanisms regulating this process are unclear. Although many physiological signals, including glucocorticoid hormones, are pulsatile, ...
Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
(Cell Press, 2016-04)
The estrogen receptor (ER), glucocorticoid receptor (GR), and forkhead box protein 1 (FoxA1) are significant factors in breast cancer progression. FoxA1 has been implicated in establishing ER-binding patterns though its ...
Controlled reduction of photobleaching in DNA origami gold nanoparticle hybrids
(American Chemical Society, 2014-04)
The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or ...
A deep learning-based approach to model anomalous diffusion of membrane proteins: The case of the nicotinic acetylcholine receptor
(Oxford University Press, 2021-10)
We present a concatenated deep-learning multiple neural network system for the analysis of single-molecule trajectories. We apply this machine learning-based analysis to characterize the translational diffusion of the ...
Pulsed Interleaved MINFLUX
(American Chemical Society, 2020-12)
We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the ...
Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
(Nature Publishing Group, 2018-12)
Translational motion of neurotransmitter receptors is key for determining receptor number at the synapse and hence, synaptic efficacy. We combine live-cell STORM superresolution microscopy of nicotinic acetylcholine receptor ...
When size does matter: organelle size influences the properties of transport mediated by molecular motors
(Elsevier Science, 2013-11)
Background: Organelle transport is driven by the action of molecular motors. In this work, we studied the dynamics of organelles of different sizes with the aim of understanding the complex relation between organelle motion ...