info:eu-repo/semantics/article
Pulsed Interleaved MINFLUX
Fecha
2020-12Registro en:
Masullo, Luciano Andrés; Steiner, Florian; Zähringer, Jonas; Lopez, Lucía Fernanda; Bohlen, Johann; et al.; Pulsed Interleaved MINFLUX; American Chemical Society; Nano Letters; 21; 1; 12-2020; 840-846
1530-6984
CONICET Digital
CONICET
Autor
Masullo, Luciano Andrés
Steiner, Florian
Zähringer, Jonas
Lopez, Lucía Fernanda
Bohlen, Johann
Richter, Lars
Cole, Fiona
Tinnefeld, Philip
Stefani, Fernando Daniel
Resumen
We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1-2 nm localization precision with 2000-1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.