Artículos de revistas
Controlled reduction of photobleaching in DNA origami gold nanoparticle hybrids
Fecha
2014-04Registro en:
Pellegrotti, Jesica Vanesa; Acuña, Guillermo; Puchkova, Anastasiya; Holzmeister, Phil; Gietl, Andreas; et al.; Controlled reduction of photobleaching in DNA origami gold nanoparticle hybrids; American Chemical Society; Nano Letters; 14; 5; 4-2014; 2831-2836
1530-6984
Autor
Pellegrotti, Jesica Vanesa
Acuña, Guillermo
Puchkova, Anastasiya
Holzmeister, Phil
Gietl, Andreas
Lalkens, Birka
Stefani, Fernando Daniel
Tinnefeld, Philip
Resumen
The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles? size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching.