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Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples
(American Society of Tropical Medicine and Hygiene, 2015)
Giardia duodenalis is one of the most commonly identified parasites in stool samples. Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we ...
Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool
(American Society of Tropical Medicine and Hygiene, 2017)
Fasciola hepatica is the most widely distributed trematode infection in the world. Control efforts may be hindered by the lack of diagnostic capacity especially in remote endemic areas. Polymerase chain reaction (PCR)-based ...
Microgeographical differences of Plasmodium vivax relapse and re-infection in the Peruvian Amazon
(American Society of Tropical Medicine and Hygiene, 2013)
To determine the magnitude of Plasmodium vivax relapsing malaria in rural Amazonia, we carried out a study in four sites in northeastern Peru. Polymerase chain reaction-restriction fragment length polymorphism of PvMSP-3α ...
Lack of molecular correlates of Plasmodium vivax ookinete development
(American Society of Tropical Medicine and Hygiene, 2011)
Previous studies of Plasmodium vivax transmission to Anopheles spp. mosquitoes have not been able to predict mosquito infectivity on the basis of microscopic or molecular quantification of parasites (total parasites in the ...
Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis
(American Society for Microbiology, 2011)
We compared traditional cutaneous leishmaniasis diagnostic methods to filter paper lesion impression (FPLI) PCR for secondarily infected ulcers and nonulcerative lesions. The sensitivity and specificity of FPLI PCR for ...
Comparative gene expression analysis throughout the life cycle of Leishmania braziliensis: Diversity of expression profiles among clinical isolates
(Public Library of Science, 2011)
Background: Most of the Leishmania genome is reported to be constitutively expressed during the life cycle of the parasite, with a few regulated genes. Inter-species comparative transcriptomics evidenced a low number of ...
Leishmania species in biopsies of patients with different clinical manifestations identified by high resolution melting and nested PCR in an Endemic district in Peru
(Elsevier, 2019)
Background: The parasite of the genus Leishmania causes a neglected disease known as leishmaniasis, which has different clinical aspects depending on the species that infects the person and the immune response of the ...
Molecular characterization of the Taenia solium Tso31 antigen and homologous of other Taenia species from Peru
(Springer Verlag, 2019)
Several studies have been performed to determine specific antigens for the diagnosis of tapeworms. One of these antigens is Tso31, which is used to differentiate Taenia solium and Taenia saginata in human feces. The aim ...
Multiplex real-time PCR for detection of Campylobacter, Salmonella, and Shigella
(American Society for Microbiology, 2013)
Infectious diarrhea can be classified based on its clinical presentation as noninflammatory or inflammatory disease. In developing countries, among inflammatory diarrhea cases, Shigella is the most common cause, followed ...
Use of kDNA-based Polymerase Chain Reaction as a Sensitive and Differentially Diagnostic Method of American Tegumentary Leishmaniasis in Disease-Endemic Areas of Northern Argentina
(American Society of Tropical Medicine and Hygiene, 2007-10)
We evaluated the polymerase chain reaction (PCR) for the diagnosis of endemic American Tegumentary Leishmaniasis in Salta, Argentina. Diverse Leishmania species, coexistence of mycotic and varicose ulcers, and high endemicity ...