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The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
(Crimson Publishers, 2020-08)
Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been ...
ImuB and ImuC contribute to UV-induced mutagenesis as part of the SOS regulon in Pseudomonas aeruginosa
(Wiley Online Library, 2019-03)
DNA damage-induced mutagenesis is a process governed by the SOS system that requires the activity of specialized DNA polymerases. These polymerases, which are devoid of proof-reading activity, serve to increase the probability ...
The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
(Nature Publishing Group, 2019-07)
Previously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms ...
Mutagénesis como técnica en la búsqueda de variabilidad genética
(Asociación para la Promoción de la Producción Algodonera, Argentina, 2020)
Las técnicas de mutagénesis o inducción de mutaciones en plantas son técnicas muy utilizadas por los fitomejoradores con el objetivo de generar diversidad genética. Esta diversidad puede afectar diversos caracteres de ...
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4-ethylphenol production
(Springer, 2021-01)
The use of non-conventional yeast species to obtain interesting flavors and aromas has become a new trend in the fermented beverages industry. Among such species, Brettanomyces bruxellensis (B. bruxellensis) has been ...
Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato
(Nature Publishing Group, 2021-02-22)
DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein ...