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Geographical clustering of Trypanosoma cruzi I groups from Colombia revealed by low-stringency single specific primer-PCR of the intergenic regions of spliced-leader genes
(Springer, 2009-01)
A low-stringency single-primer polymerase chain reaction (LSSP-PCR) typing procedure targeted to the intergenic regions of spliced-leader genes (SL) was designed to profile Trypanosoma cruzi I stocks from endemic regions ...
Transmission dynamics of Trypanosoma cruzi determined by low-stringency single primer polymerase chain reaction and southern blot analyses in four indigenous communities of the Sierra Nevada de Santa Marta, Colombia
(2009)
This study attempted to evaluate the transmission dynamics of Trypanosoma cruzi in four indigenous communities of Sierra Nevada de Santa Marta (SNSM), Colombia. Low-stringency single primer-polymerase chain reaction ...
Trypanosoma cruzi: An analysis of the minicircle hypervariable regions diversity and its influence on strain typing
(Elsevier, 2008-11)
The current intraspecific nomenclature in Trypanosoma cruzi describes two major lineages, named T. cruzi I and T. cruzi II, and five sublineages within T. cruzi II, named IIa, IIb, IIc, IId and IIe. The polymorphism of ...
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila
(2018-05-01)
In this study we analyzed the genetic variability of lipase gene sequences from eight oil and grease-degrading
strains of Candida palmioleophila and to relate it to their degrading ability. The genetic variability of ...
RT-PCR and Dot Blot hybridization methods for a universal detection of tospovirusesMétodos de RT-PCR e de hibridização Dot Blot para detecção universal de tospovirus
(Sociedade Brasileira de Fitopatologia, 2001)
Transcriptase reverse - polymerase chain reaction (RT-PCR) and dot blot hybridization with digoxigenin-labeled probes were applied for the universal detection of Tospovirus species. The virus species tested were Tomato ...
kDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patients
(Elsevier B.V., 2008-11-01)
Trypanosoma cruzi presents a high degree of intraspecific variability, with possible implications for the pathogenesis of Chagas disease. The aim of this study was to evaluate T cruzi kDNA minicircle gene signatures using ...
kDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patients
(Elsevier B.V., 2008-11-01)
Trypanosoma cruzi presents a high degree of intraspecific variability, with possible implications for the pathogenesis of Chagas disease. The aim of this study was to evaluate T cruzi kDNA minicircle gene signatures using ...
Avaliação da combinação de diferentes métodos laboratoriais utilizados para diagnosticar e caracterizar Leptospira spp. a partir de amostras de sangue de pacientes com suspeita de leptospirose em Minas Gerais, Brasil, 2008 a 2014
(Universidade Federal de Minas GeraisUFMG, 2016-06-22)
Introduction: Leptospirosis is an infectious and acute disease caused by leptospira spp. The disease has variable clinical features and is difficult to diagnose. Therefore, laboratory and epidemiological approaches are ...