Artículo de revista
Molecular detection and characterization of novel lipase genes of the lipolytic yeast Candida palmioleophila
Fecha
2018-05-01Registro en:
Rodriguez-Mateus Z., Vera Pacheco K., Zafra G., 2018, Molecular detection and characterization of novel lipase
genes of the lipolytic yeast candida palmioleophila, Chemical Engineering Transactions, 64, 349-354 DOI: 10.3303/CET1864059
10.3303/CET1864059
978-88-95608-56-3
2283-9216
Autor
Rodríguez Mateus, Zully
Vera Pacheco, Katerine
Zafra, German
Institución
Resumen
In this study we analyzed the genetic variability of lipase gene sequences from eight oil and grease-degrading
strains of Candida palmioleophila and to relate it to their degrading ability. The genetic variability of lipase
genes was analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) and low-stringency single
specific primer- PCR (LSSP-PCR), in order to obtain specific DNA fingerprints from each strain, which were
subsequently compared by bioinformatic programs. DNA fingerprints were contrasted to the ability of each
strain to remove palm oil in liquid culture. The results showed that at least three genes encoding lipases are
present in C. palmioleophila, two of them resembling the LIP2 and LIP6 genes of C. albicans. DNA fingerprints
obtained by LSSP-PCR revealed differences in the sequences of C. palmioleophila lipase genes, which
allowed to group the strains according to their degrading activity. C. palmioleophila strains SACL05, SACL08
and SACL11, which showed the highest removal of palm oil after 72 h (77 to 79 % removal), were grouped in
a single clade in dendrograms. Similarly, strains SACL01, SACL03, SACL06 and SACL09, which showed
intermediate removal activity of palm oil (54 to 76%) grouped in a different clade. This suggests the genetic
variability in lipase genes is directly related to the differences found in the efficiency of degradation of oils. On
the other hand, DNA fingerprints obtained by PCR-RFLP did not allow to differentiate the strains and did not
generate changes in the bands patterns between the analyzed strains. In conclusion, this study reported for
the first time the detection and characterization of lipase genes from the lipolytic yeast Candida
palmioleophila, and their association to the degradation of oils.