Article
Genetic polymorphism in Trypanosoma cruzi I isolated from Brazilian Northeast triatomines revealed by low-stringency single specific primer–polymerase chain reaction
Registro en:
BRITO, C. M. M. et al. Genetic polymorphism in Trypanosoma cruzi I isolated from Brazilian Northeast triatomines revealed by low-stringency single specific primer–polymerase chain reaction. Parasitol Res., v.103, p.1111–1117, 2008.
0932-0113
1432-1955
Autor
Brito, C. M. M.
Lima, Marli Maria
Sarquis, O.
Pires, M. Q.
Coutinho, C. F. S.
Duarte, R.
Pacheco, R. S.
Resumen
Different molecular markers have been employed
for typing Trypanosoma cruzi strains from endemic areas of
Chagas disease. The low-stringency single specific primer–
polymerase chain reaction (LSSP–PCR) has been a sensitive
and informative technique that uses the variable
region of kinetoplast DNA minicircles as a genetic marker,
allowing detection of DNA sequence variation. In the
present study, we analyzed the intra-lineage genetic
variability of the T. cruzi strains obtained from triatomine
feces collected on filter paper FTA card by LSSP–PCR.
The hybridization of the PCR products with a probe for the
subgenus Schizotrypanum and a clone-specific probe from
Dm28c confirmed the subgenus as T. (S.) cruzi and
respective lineages as T. cruzi I. Phenetic analysis showed
the presence of three clusters that diverged by different
coefficients of similarity. Thirteen T. cruzi I genotypes were
observed circulating among Triatoma pseudomaculata and
Rhodnius nasutus from peridomiciliary and natural environments
in five peri-urban and urban localities of Jaguaruana,
Ceará, Brazil. These data indicate the importance of the
circulation of T. cruzi I genotypes among T. pseudomaculata
and R. nasutus in different environments and the possible
risk of Chagas disease domestic transmission. 2030-01-01