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A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
(John Wiley & Sons Ltd, 2009-05)
Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated ...
Strategy for erythroid differentiation in ex vivo cultures: Lentiviral genetic modification of human hematopoietic stem cells with erythropoietin gene
(Soc Bioscience Bioengineering Japan, 2017-11)
If cultured in appropriate conditions, such as supplementing culture media with costly cytokines and growth factors, hematopoietic stem/progenitor cells (HSPCs) from different origins have shown to be an adequate source ...
Measles virus envelope pseudotyped lentiviral vectors transduce quiescent human HSCs at an efficiency without precedent
(American Society of Hematology, 2017-10)
Hematopoietic stem cell (HSC)–based gene therapy trials are now moving toward the use of lentiviral vectors (LVs) with success. However, one challenge in the field remains: efficient transduction of HSCs without compromising ...
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
(BioMed Central Ltd., 2018-07)
Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this ...
Baboon envelope pseudotyped LVs outperform VSV-G-LVs for gene transfer into early-cytokine-stimulated and resting HSCs
(American Society of Hematology, 2014-08)
Hematopoietic stem cell (HSC)-based gene therapy holds promise for the cure of many diseases. The field is now moving toward the use of lentiviral vectors (LVs) as evidenced by 4 successful clinical trials. These trials ...
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
(BioMedCentral, 2018-07-27)
OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this ...
Development of a suitable manufacturing process for production of a bioactive recombinant equine chorionic gonadotropin (reCG) in CHO-K1 cells
(Elsevier Science Inc., 2021-09)
Equine chorionic gonadotropin (eCG) is a heterodimeric glycoprotein hormone produced by pregnant mares that has been used to improve reproductive performance in different domestic species. Several strategies to produce the ...
Silencing brain catalase expression reduces ethanol intake in developmentally-lead-exposed rats
(Elsevier Science, 2019-06)
Lead (Pb) is a developmental neurotoxicant. We have demonstrated that perinatally Pb-exposed rats consumemore ethanol than their control counterparts, a response that seems to be mediated by catalase (CAT) andcentrally-formed ...
Generation of a human induced pluripotent stem cell line from a familial Alzheimer's disease PSEN1 T119I patient
(Elsevier, 2021-05)
Human induced pluripotent stem cells (hiPSC) line FLENIi001-A was reprogrammed from dermal fibroblasts using the lentiviral-hSTEMCCA-loxP vector. Fibroblasts were obtained from a skin biopsy of a 72-year-old Caucasian male ...
Silencing of cardiac mitochondrial NHE1 prevents mitochondrial permeability transition pore opening
(American Physiological Society, 2011-04)
Inhibition of Na +/H + exchanger 1 (NHE1) reduces cardiac ischemia-reperfusion (I/R) injury and also cardiac hypertrophy and failure. Although the mechanisms underlying these NHE1-mediated effects suggest delay of mitochondrial ...