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Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
(Elsevier Science, 2016-01)
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency ...
Linear peptide analogues from Jatropha's orbitides promote migration of human neonatal foreskin fibroblasts in vitro and collagen deposition
(2019-01-01)
Orbitides are a class of small naturally occurring cyclic peptides with structural and functional diversities. Their chemical properties make this class feasible to be obtained by solid phase peptide synthesis (SPPS). ...
Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
(Elsevier, 2021)
KISCOi001-A is a healthy feeder-free and fully characterized human induced pluripotent stem (iPS) cell line cultured under xeno-free and defined conditions. The cell
line is generated from normal human foreskin fibroblasts ...
Activation of β 3 adrenergic receptor decreases DNA synthesis in human skin fibroblasts via cyclic GMP/nitric oxide pathway
(Karger, 2005-07)
Background: Evidences have shown that β 1 and β 2 adrenoceptors co-exist in human fibroblasts, but it is not yet clear the functional expression of β 3 adrenoceptor in these cells. The aim of this study was to investigate ...
Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
(2018-10-30)
Skin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and ...
Regulation of cyclin E1 expression in human pluripotent stem cells and derived neural progeny
(Landes Bioscience, 2018-07)
Human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem cells (hESCs and hiPSCs) show unique cell cycle characteristics, such as a short doubling time due to an abbreviated G1 phase. Whether ...
Thermolysin in human cultured keratinocyte isolation
(Instituto Internacional de Ecologia, 2007-02-01)
BACKGROUND: When treating extensively burned patients using cultured epidermal sheets, the main problem is the time required for its production. Conventional keratinocyte isolation is usually done using Trypsin. We used a ...
Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
(Universidade Federal de UberlândiaBrasilBiomedicina, 2018)