Artículos de revistas
Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
Fecha
2021Registro en:
Stem Cell Research 51 (2021) 102193 Available
10.1016/j.scr.2021.102193
Autor
Inzunza, José
Arias Fuenzalida, Jonathan Lee
Segura Aguilar, Juan Ernesto
Nalvarte, Iván
Varshney, Mukesh
Institución
Resumen
KISCOi001-A is a healthy feeder-free and fully characterized human induced pluripotent stem (iPS) cell line cultured under xeno-free and defined conditions. The cell
line is generated from normal human foreskin fibroblasts with non-integrating episomal plasmid vectors encoding OCT4, SOX2, KLF4, NANOG, LIN28, nontransforming
L-MYC and dominant negative p53. The generated iPS cells are transgene-free and their pluripotency is confirmed by the expression of stem cell markers
and capacity to differentiate into the cells of ectoderm, endoderm and mesoderm while their identity and karyotype stability is confirmed with Genomic assays.