info:eu-repo/semantics/article
Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
Fecha
2016-01Registro en:
Questa, María; Romorini, Leonardo; Bluguermann, Carolina; Solari, Claudia María; Neiman, Gabriel; et al.; Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts; Elsevier Science; Stem Cell Research; 16; 2; 1-2016; 300-303
1873-5061
CONICET Digital
CONICET
Autor
Questa, María
Romorini, Leonardo
Bluguermann, Carolina
Solari, Claudia María
Neiman, Gabriel
Luzzani, Carlos Daniel
Scassa, Maria Elida
Sevlever, Gustavo
Guberman, Alejandra Sonia
Miriuka, Santiago Gabriel
Resumen
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.