Now showing items 1-10 of 248
A Practical Teaching Course in Directed Protein Evolution Using the Green Fluorescent Protein as a Model
Protein engineering is a powerful tool, which correlates protein structure with specific functions, both in applied biotechnology and in basic research. Here, we present a practical teaching course for engineering the green ...
Citrate and Phosphate Influence on Green Fluorescent Protein Thermal Stability
(JOHN WILEY & SONS LTD, 2011)
Protein structure and function can be regulated by no specific interactions, such as ionic interactions in the presence of salts. Green fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; ...
Effect of Polyethylene Glycol on the Thermal Stability of Green Fluorescent Protein
(JOHN WILEY & SONS LTD, 2010)
Green fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; its stability can be directly related to its fluorescence output, among other characteristics. GFP is stable under increasing ...
Protein refolding at high pressure: Optimization using eGFP as a model
(Elsevier Sci LtdOxfordInglaterra, 2011)
Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds
BACKGROUND: Green fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) ...
Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein
(Korean Soc Parasitology, Seoul Natl Univ Coll Medi, 2011-12-01)
Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania ...
Investigation of charged polymer influence on green fluorescent protein thermal stability
(ELSEVIER SCIENCE BV, 2011)
Methods of stabilization and formulation of proteins are important in both biopharmaceutical and biocatalysis industries. Polymers are often used as modifiers of characteristics of biological macromolecules to improve the ...