Buscar
Mostrando ítems 1-10 de 316
β-cyclodextrin modifications as related to enzyme stability in dehydrated systems: Supramolecular transitions and molecular interactions
(Elsevier, 2011-01)
The effect of β-cyclodextrin modifications (polymerization (PCD) and later carboxymethylation (CMPCD)) on their action as enzyme stabilizers was analyzed during freeze-drying and thermal treatment. Combined polymer-trehalose ...
Beyond Stability Constraints: A biophysical model of enzyme evolution with selection on stability and activity
(Oxford University Press, 2018-12-27)
The rate of evolution varies among sites within proteins. In enzymes, two rate gradients are observed: rate decreases with increasing local packing and it increases with increasing distance from catalytic residues. The ...
Catalase Stability in Amorphous and Supercooled Media Related to Trehalose- Water- Salt Interactions
(Springer, 2019-03)
Sugars are the most common excipients added to pharmaceutical and biotechnological formulations as protein protectants due to their adequate physicochemical properties, low toxicity, high purity and low cost. However, a ...
Stabilizing effects of cations on lipases depend on the immobilization protocol
(Royal Society of Chemistry, 2015-09)
The effect of an additive on enzyme stability used to be considered an intrinsic feature of a lipase. However, in this paper we have found that the effect of additive on enzyme stability depends on the immobilization ...
Kinetic stability of membrane proteins
(Springer Verlag, 2017-10)
Although membrane proteins constitute an important class of biomolecules involved in key cellular processes, study of the thermodynamic and kinetic stability of their structures is far behind that of soluble proteins. It ...
A Peptidase Enzyme from Bacillus cereus with Antimicrobial Properties: Optimizing the Immobilization in Chitosan Beads Using Box-Behnken Design
(Sciepub, 2016-05)
Enzymes are exploited as catalysts in many industrial, biomedical, and analytical processes. There has been considerable interest in the development of carrier systems for enzyme immobilization because immobilized enzymes ...
Structural and molecular dynamics investigations of ligand stabilization via secondary binding site interactions in Paenibacillus xylanivorans GH11 xylanase
(Elsevier, 2021-03)
Glycoside hydrolases (GHs) are essential for plant biomass deconstruction. GH11 family consist of endo-β-1,4-xylanases which hydrolyze xylan, the second most abundant cell wall biopolymer after cellulose, into small ...
Polyethylene glycol-based low generation dendrimers functionalized with β-cyclodextrin as cryo- and dehydro-protectant of catalase formulations
(American Chemical Society, 2013-05)
Polyethylene glycol (PEG)-based low generation dendrimers are analyzed as single excipient or combined with trehalose in relation to their structure and efficiency as enzyme stabilizers during freeze-thawing, freeze-drying, ...
New insights on enzyme stabilization for industrial biocatalysis
(American Chemical SocietyUnited States, 2022)
Triosephosphate isomerase deficiency: effect of F240L mutation on enzyme structure
(Elsevier Science Inc., 2020-08-15)
Eleven missense mutations have been describe in human triosephosphate isomerase (TPI), affecting its catalytic function. Several of these mutations generate triosephosphate isomerase deficiency, the consequences of which ...